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Cat#: VRS-0124-YT5233
Lenti-CHIKV E3/E2/6k/E1 Pseudovirus [Luciferase, HIV-1 Packaging System]
CHIKV E3/E2/6k/E1 pseudovirus is produced by cotransfecting 293T cells with the plasmid encoding CHIKV envelope protein, pDR8.2 (HIV gag-pol), and pTrip Luciferase. Pseudovirus-containing supernatant was harvested 24 h following transfection. The supernatant was cleared of cell debris by centrifugation and filtered through a 0.45-μm-pore-size filter. The produced pseudoviruses carry CHIKV E3/E2/E1 proteins, which is a powerful tool for CHIKV vaccine and entry inhibitor research.
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- Product Summary
- Packaging, Storage & Formulations
- Target Details
Product Summary
| Type : | Pseudovirus |
| Virus Name : | Chikungunya Virus |
| Virus Packaging Protein : | E3/E2/6k/E1 |
| Packaging System : | HIV-1 Packaging System |
| Packaging Cell : | 293T |
| Reporter Gene : | Luciferase |
| Titer : | >1x 108 virus copies/mL |
| Application Notes : | The pseudovirus can be usd for the following applications: 1. Antibody neutralization assay, Serum neutralization assay. 2. As virus standard alternative in diagnostic assay or others. 3. Drug screening including vaccine development, viral entry inhibitor development. 4. As a safe virology tool to discover the virus entry mechanisms. |
Packaging, Storage & Formulations
| Form : | Liquid |
| Storage : | Store at -80°C and stable for 6 months from the date of shipment, avoiding freeze/thaw cycles. |
| Shipping : | Dry ice |
| Note : | The pseudovirus can be used under BSL-2 conditions. Please reach out to our scientists for detailed instructions or guidelines of the pseudovirus. |
Target Details
| Introduction : | Chikungunya virus (CHIKV) is an alphavirus belonging to the Togaviridae family. It has a positive-sense single-stranded RNA genome with 11.8 kb long. The genome encodes five structural proteins, the capsid and the envelope proteins (E3, E2, 6k, and E1). E2 and E1 form a heterodimer that projects as an outward-facing spike on the surface of CHIKV particles. E2 protein regulates the virus attachment to cell receptor and E1 may be responsible for promoting the release of the viral nucleocapsid. E3 glycoprotein is associated with mature virions in some alphaviruses. 6K protein is incorporated into particles at a low level andnappears to potentially have multiple roles in glycoprotein processing, cell permeabilization, and viral budding. |
| Alternative Names : | E3/E2/6k/E1; CHIKV envelope protein; CHIKVgp2; CHIKV structural polyprotein |
| Gene ID : | 956308 |
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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