Secondary antibodies can bind to the primary antibodies which can help amplify the signal intensity in an immunoassay. Creative Biolabs is dedicated to providing reagents and services to help understand and accelerate infectious disease research. With rich experience in antibody development and manufacturing, we provide both primary antibodies and secondary antibodies to enable you to find the perfect solution for your virology research purposes. We have secondary antibodies with different conjugates for various immunoassays, such as western blots, flow cytometry, ELISA, and immunohistochemistry, etc.
Our secondary antibodies by species reactivity
Our secondary antibodies by host species
If you don't find a secondary antibody you want, please contact us and our clients will help you find the best secondary antibody to meet your needs.
Tips for Choosing the Best Secondary Antibody
To successfully choose a secondary antibody that is best for your application and research, consider the following factors:
- Reactivity
Depending on the host species of your primary antibody.
- Specificity
Depending on the class/subclass of your primary antibody.
- Conjugation
Depending on the intended application and how the secondary antibody is going to be detected.
- Format
Consider using whole antibodies or fragmented secondary antibodies (Fab and F(ab')2) in certain situations.
- Cross-reactivity
Choosing a pre-adsorbed secondary antibody to eliminate non-specific, background binding and cross-reactivity.
Conjugations and Applications
A secondary antibody is usually conjugated with enzymes, biotin, and fluorescent dyes/proteins. The conjugate choice depends on the detection method and experimental application. For WB and ELISA assays, enzyme-labeled secondary antibodies such as HRP and AP are usually used, and both can be detected using colorimetric and chemiluminescent platforms. For specific conjugation, please contact us. We also provide custom conjugation services at optimized incorporation ratios.
- Immunoassay Experiment
- Secondary Antibody Label
- Western blotting
- HRP, AP, Fluorescent Dyes
- ELISA
- HRP or Biotin
- Flow Cytometry
- Alexa Fluor, Phycoerythrin, FITC
- Immunohistochemistry
- Biotin, AP, HRP or HRP-polymer
- Immunocytochemistry, IHC-Fr
- Alexa Fluor, DyLight
Our Competitiveness
At Creative Biolabs, we have more than 10,000 high-quality antibodies for our customers to accelerate their virology research. We provide free support to guide customers to select the correct reagents for their research needs. To respond to requests from customers, our products undergo continuous updates to make sure that they find exactly what they need for their research. All of our antibodies are rigorously tested and validated to meet our rising standards and commitment to antibody performance.
Antibody Discovery Service
Based on a pepFastX™ Platform, we offer monoclonal antibody discovery services to help our clients accelerate their virology research projects. Our experienced technical experts are available to introduce the technology and help shorten your project timeline. If you want to achieve the goal of fast antibody discovery, please get in touch with us for more information.