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Hemagglutination Inhibition Assay

Creative Biolabs has developed a variety of virology assays to determine viral titer and test the efficacy of vaccines and drugs based on an extensive versatile technical platform. The hemagglutination assay is a well-established and reproducible method for characterizing viruses such as influenza viruses antigenically. Our experienced scientists can provide hemagglutination assay to help you complete your virology studies faster.

Introduction

Hemagglutination is a reaction that causes the clumping of red blood cells (RBCs) in presence of some enveloped viruses. The hemagglutination assay and the hemagglutination inhibition assay created to measure the relative concentration of viruses, bacteria, or antibodies. In the absence of virus particles, RBCs do not clump and instead form a red-colored dot at the bottom of a container. RBCs clump together in the presence of virus particles due to interaction between virus particle hemagglutinin (HA) proteins and RBCs, resulting in the formation of a lattice. A red dot does not form in this case. The formation of the lattice is determined by the virus and RBC concentrations. The hemagglutination inhibition assay is based on the idea that antibodies to that particular virus will prevent the virus from attaching to RBCs. As a result, when antibodies are present, hemagglutination is inhibited.

Principle of the haemagglutination assay and haemagglutination inhibition assay.Fig.1 Principle of the haemagglutination assay and haemagglutination inhibition assay. (Kaufmann, 2017)

What Can We offer?

Creative Biolabs, with extensive expertise and experience in the field of virology, offers both hemagglutination assay and hemagglutination inhibition assay. Viral samples are serially diluted and dispensed into each well of a 96-well microtiter plate to perform the hemagglutination assay. Following that, RBC aliquots are added to each well. The virus-free well serves as a negative control. A standardized amount of virus is mixed with serially diluted antisera in a 96-well microtiter plate for the hemagglutination inhibition test. Then RBCs are added to determine the antibody's specific binding to the HA molecule.

Our hemagglutination assay is commonly used to detect viral infections caused by

  • Orthomyxoviruses (influenza)
  • Paramyxoviruses (measles, mumps)
  • Mononucleosis
  • Arboviruses-togaviruses (including rubella)
  • Flaviviruses
  • Bunyaviruses

Creative Biolabs is a virus research consultancy and experimental service provider. With many years of experience, we can provide you with more solutions, increase efficiencies, and shorten your drug development timelines. We are delighted to make it available to a wide range of research and industrial clients. Please contact us for more information if you are interested in the services we offer.

Reference

  1. Kaufmann, L.; et al. An optimized hemagglutination inhibition (HI) assay to quantify influenza-specific antibody titers. JoVE (Journal of Visualized Experiments), 2017 (130): e55833.

All products and services are intended for Research Use Only, and NOT to be used in diagnostic or therapeutic procedures.

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