An isotype control is a primary antibody as a negative control that matches the isotypes of the experimental antibody in standard immunoassays. The only difference between the isotype control and experimental primary antibody is antigen specificity: isotype control has no specificity for a target antigen/cell. Therefore, isotype controls help differentiate non-specific background signals caused by off-target binding. With years of experience in antibody development, manufacturing, and engineering, Creative Biolabs provides various types of isotype control antibodies for a variety of research applications such as flow cytometry and immunohistochemistry. Our isotype control products have been fully characterized and validated to avoid producing misleading results. Designed with our customer's research needs in mind, our products are available as unconjugated or conjugated to biotin, HRP, and fluorophores. You can trust our high-quality isotype controls to support your research needs across various applications.
Fig.1 Comparison of the primary antibody and
isotype control
Our Isotype antibodies by host species:
The isotype control should be matched to the characteristics of the experimental antibody including host species, isotype, and conjugates. We are always here to hear from our customers! If you do not find the desired isotype controls, please contact us for custom assistance.
Key Features
For the convenience of our customers, Creative Biolabs provides a wide range of isotype control antibodies to meet clients' research requirements. Our isotype controls feature:
- Extensive selection of various isotope controls.
- Strict quality control to ensure good customer experience.
- High consistency across batches.
- Exceptional purity.
Tips for Choosing the Best Isotype Controls
The most appropriate isotype control matches certain characteristics:
- No binding to the target of interest.
- The same host species as the primary antibody.
- The same Ig class and subclass.
- The same conjugate as the primary antibody.
For example, if your primary antibody is a human (IgG1, kappa) Anti-SARS-COV-2 spike-PE, you will need a human IgG1, kappa-PE isotype control. Besides, the same testing conditions are necessary when making comparisons, including same samples, working concentration, incubation, and detection method.
Applications of Isotype Controls
The isotype control is a necessary tool in standard immunoassays. Appropriate isotype control is important for the accurate interpretation of study results. Our isotype controls are available in diverse species, antibody subclass, and conjugation, making them suitable for a broad variety of applications:
- Western Blotting (WB)
- Enzyme-linked immunosorbent assay (ELISA)
- Flow cytometry (FC)
- Immunohistochemistry (IHC)
- Immunocytochemistry (ICC)