Documents
Cat#: VRS-0224-YT877
ViroAb™ Chimeric (Mouse/Rabbit)Anti-HIV PR Monoclonal Antibody (XS0061)
This Chimeric (Mouse/Rabbit) Monoclonal antibody is specific for HIV PR protein. The antibody can be used for applications: WB, ELISA, Neut. The antibody is for Acquired Immune Deficiency Syndrome disease research use only and is not approved for use in humans or in clinical diagnosis.
Made to Order Requirements
Your choose infomation:
CLEAR(Note: Please select the requirements to fit you project. If you have any other requirements, please specify. If no specific requirements, you don't need to select any button.)
- Product Overview
- Product Properties
- Packaging, Storage & Formulations
- Applications
Product Overview
| Target : | PR |
| Specificity : | 1696 binds to HIV-PR of both HIV-1 and HIV-2. The antibody binds to the N-terminal region of the enzyme, and binding is inhibited by the peptide fragment PQITLWQ which represents residues 1-7. WB binding assays show that 1696 recognizes the mature and processed form of the enzyme, but not the precursor which possesses 20 residues upstream from the cleavage site. The N-terminus has a well conserved structure between HIV-1 PR and HIV-2 PR. HIV-PR is a homodimeric aspartate protease that specifically cleaves the viral Gag and Gag/Pol polyprotein precursor. A correlation has been observed between the activity of the protease and the degree of infectivity, and so this protein is a major antiviral target. |
| Clone : | XS0061 |
| Host Species : | Chimeric (Mouse/Rabbit) |
| Antibody Isotype : | IgG1, kappa |
| Species Reactivity : | Human Immunodeficiency Virus 1; Human Immunodeficiency Virus 2 |
Product Properties
| Immunogen : | 1696 was prepared by generating a hybridoma cell line, fusing myeloma cells with splenocytes derived from BALB/c mice immunized with HIV-1 PR in the presence of Freund's adjuvant. |
| Purity : | >95% as determined by SDS-PAGE |
| Purification : | Protein A affinity chromatography |
| Concentration : | Lot specific |
Packaging, Storage & Formulations
| Form : | Liquid |
| Formulation : | PBS, 0.02% Proclin 300 |
| Preservative : | 0.02% Proclin 300 |
| Storage : | Store at 4°C for short term. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
Applications
| Application : | WB; ELISA; Neut |
| Application Notes : | 1696 mAb can inhibit the catalytic activity of both HIV-1 and HIV-2 HIV-PR. Inhibition by 1696 is mediated by the destabilization of the of the active form of the active HIV-PR homodimer through binding to the N-terminus, which constitutes a large percentage of the interface between the monomers. The Fab fragment was generated by incubation of the antibody with pepsin. Binding constants of 1696 can be determined by ELISA. 1696 shows reactivity to the mature processed HIV-PR and not to the precursor by WB. |
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
pepFastX™ Platform
If no antibody is available for your research, we recommend our novel pepFastX™ antibody discovery platform. The mAb products can be delivered within 2-3 months.
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support