Retro-SARS-CoV Spike Pseudovirus [EGFP, HIV-1 Packaging System]

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Cat#: VRS-0124-YT5230

Retro-SARS-CoV Spike Pseudovirus [EGFP, HIV-1 Packaging System]

SARS-CoV spike pseudovirus is produced by cotransfecting HEK293 cells with Pqcxix that has been engineered to insert an EGFP gene, SARS-CoV spike protein-encoding vector, and gag/pol expression plasmid using Lipofectamine 2000 (Invitrogen). Pseudovirus-containing supernatant was harvested 48 h following transfection. The supernatant was cleared of cell debris by centrifugation and filtered through a 0.45-μm-pore-size filter.

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  • Product Summary
  • Packaging, Storage & Formulations
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Product Summary

Type : Pseudovirus
Virus Name : Severe Acute Respiratory Syndrome Coronavirus
Virus Packaging Protein : Spike
Packaging System : HIV-1 Packaging System
Packaging Cell : HEK293
Reporter Gene : GFP
Titer : >1x 108 virus copies/mL
Application Notes : The pseudovirus can be usd for the following applications:
1. Antibody neutralization assay, Serum neutralization assay.
2. As virus standard alternative in diagnostic assay or others.
3. Drug screening including vaccine development, viral entry inhibitor development.
4. As a safe virology tool to discover the virus entry mechanisms.

Packaging, Storage & Formulations

Form : Liquid
Storage : Store at -80°C and stable for 6 months from the date of shipment, avoiding freeze/thaw cycles.
Shipping : Dry ice
Note : The pseudovirus can be used under BSL-2 conditions. Please reach out to our scientists for detailed instructions or guidelines of the pseudovirus.

Target Details

Introduction : Severe acute respiratory syndrome (SARS) is a viral respiratory disease of zoonotic origin caused by SARS-CoV or SARS-CoV-1 infections. SARS-CoV is an enveloped, positive-sense, single-stranded RNA virus. The virus enters the host cell by the binding to the human angiotensin-converting enzyme 2 (ACE2) receptor.
Alternative Names : SARS-CoV, SARS, S, Spike, Pseudovirus, Spike Pseudovirus, S Pseudovirus, SARS-CoV Spike Pseudovirus, SARS-CoV S Pseudovirus, SARS Spike Pseudovirus, SARS S Pseudovirus
Gene ID : 1489668

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.


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