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Cat#: VRS-0124-YT5244
Lenti-EBOV GP Pseudovirus [EGFP, VSV Packaging System]
EBOV GP Pseudovirus is produced by cotransfecting 293T cells with pcDNA3.1 plasmid expressing EBOVΔO GP using the calcium phosphate transfection procedure. Cells were rinsed with phosphate-buffered saline (PBS) 12 h later to remove the transfection reagents. At 24 h following transfection, cells were transduced with VSVG pseudotyped VSV G-eGFP. Viral inoculum was removed 12 h later, and supernatants were collected at 24 h following transduction for viral stocks. The supernatant was cleared of cell debris by centrifugation and filtered through a 0.45-μm-pore-size filter. The produced pseudoviruses carry EBOV GP proteins, which is a powerful tool for EBOV glycoprotein function and entry inhibitor research.
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- Product Summary
- Packaging, Storage & Formulations
- Target Details
Product Summary
| Type : | Pseudovirus |
| Virus Name : | Ebola Virus |
| Virus Packaging Protein : | GP |
| Packaging System : | HIV-1 Packaging System |
| Packaging Cell : | 293T |
| Reporter Gene : | GFP |
| Titer : | >1x 108 virus copies/mL |
| Application Notes : | The pseudovirus can be usd for the following applications: 1. Antibody neutralization assay, Serum neutralization assay. 2. As virus standard alternative in diagnostic assay or others. 3. Drug screening including vaccine development, viral entry inhibitor development. 4. As a safe virology tool to discover the virus entry mechanisms. |
Packaging, Storage & Formulations
| Form : | Liquid |
| Storage : | Store at -80°C and stable for 6 months from the date of shipment, avoiding freeze/thaw cycles. |
| Shipping : | Dry ice |
| Note : | The pseudovirus can be used under BSL-2 conditions. Please reach out to our scientists for detailed instructions or guidelines of the pseudovirus. |
Target Details
| Introduction : | Ebola viruses (EBOVs) are the cause of repeated outbreaks of fatal infections, including the recent deadly epidemic in West Africa. The EBOV has a membrane envelope decorated by trimers of a glycoprotein (GP) which is cleaved by furin to form GP1 and GP2 subunits). GP is solely responsible for host cell attachment, endosomal entry and membrane fusion, thus becoming a primary target for the development of antiviral drugs. |
| Alternative Names : | GP; GP1,2, Envelope glycoprotein; EBOV structural polyprotein |
| Gene ID : | 911829 |
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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