Lenti-EBOV GP Pseudovirus [Luciferase, HIV-1 Packaging System]



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Cat#: VRS-0124-YT5238

Lenti-EBOV GP Pseudovirus [Luciferase, HIV-1 Packaging System]

EBOV GP Pseudovirus is produced by cotransfecting 293T cells with the plasmid encoding EBOV GP protein, pCMVΔR8.2, and a luciferase reporter plasmid. Pseudovirus-containing supernatant was harvested 24 h following transfection. The supernatant was cleared of cell debris by centrifugation and filtered through a 0.45-μm-pore-size filter. The produced pseudoviruses carry EBOV GP proteins, which is a powerful tool for EBOV vaccine and entry inhibitor research.

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Product Summary

Type : Pseudovirus
Virus Name : Ebola Virus
Virus Packaging Protein : GP
Packaging System : HIV-1 Packaging System
Packaging Cell : 293T
Reporter Gene : Luciferase
Titer : >1x 108 virus copies/mL
Application Notes : The pseudovirus can be usd for the following applications:
1. Antibody neutralization assay, Serum neutralization assay.
2. As virus standard alternative in diagnostic assay or others.
3. Drug screening including vaccine development, viral entry inhibitor development.
4. As a safe virology tool to discover the virus entry mechanisms.

Packaging, Storage & Formulations

Form : Liquid
Storage : Store at -80°C and stable for 6 months from the date of shipment, avoiding freeze/thaw cycles.
Shipping : Dry ice
Note : The pseudovirus can be used under BSL-2 conditions. Please reach out to our scientists for detailed instructions or guidelines of the pseudovirus.

Target Details

Introduction : Ebola viruses (EBOVs) are the cause of repeated outbreaks of fatal infections, including the recent deadly epidemic in West Africa. The EBOV has a membrane envelope decorated by trimers of a glycoprotein (GP) which is cleaved by furin to form GP1 and GP2 subunits). GP is solely responsible for host cell attachment, endosomal entry and membrane fusion, thus becoming a primary target for the development of antiviral drugs.
Alternative Names : GP; GP1,2, Envelope glycoprotein; EBOV structural polyprotein
Gene ID : 911829

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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