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Cat#: VRS-0124-YT5291
Retro-VSV-G Pseudovirus [HIV Packaging System]
VSV-G pseudovirus is produced by cotransfecting HEK293 cells with plasmid Pqcxix, the plasmid encoding VSV G glycoprotein, and gag/pol expression plasmid using Lipofectamine 2000 (Invitrogen). Pseudovirus-containing supernatant was harvested 48 h following transfection. The supernatant was cleared of cell debris by centrifugation and filtered through a 0.45-μm-pore-size filter.
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- Product Summary
- Packaging, Storage & Formulations
- Target Details
Product Summary
| Type : | Pseudovirus |
| Virus Name : | Vesicular stomatitis virus |
| Virus Packaging Protein : | GP |
| Packaging System : | HIV-1 Packaging System |
| Packaging Cell : | HEK293 |
| Titer : | >1x 108 virus copies/mL |
| Application Notes : | The pseudovirus can be usd for the following applications: 1. Antibody neutralization assay, Serum neutralization assay. 2. As virus standard alternative in diagnostic assay or others. 3. Drug screening including vaccine development, viral entry inhibitor development. 4. As a safe virology tool to discover the virus entry mechanisms. |
Packaging, Storage & Formulations
| Form : | Liquid |
| Storage : | Store at -80°C and stable for 6 months from the date of shipment, avoiding freeze/thaw cycles. |
| Shipping : | Dry ice |
| Note : | The pseudovirus can be used under BSL-2 conditions. Please reach out to our scientists for detailed instructions or guidelines of the pseudovirus. |
Target Details
| Introduction : | Vesicular stomatitis virus (VSV) is a bullet-shaped, single-stranded negative sense RNA virus and belongs to the family Rhabdoviridae, genus Vesiculovirus. VSV structure is a virus. Structurally, it is composed of a lipid-glycoprotein envelope surrounding the nucleoprotein core. The envelope is composed of glycoprotein (G) protein and matrix (M) protein. G protein mediates viral attachment to an LDL receptor (LDLR) or an LDLR family member present on the host cell. M protein not only is a structural protein but likely responsible for the pathogenesis by inhibiting innate interferon responses. |
| Alternative Names : | VSV-G; VSIVgp4 glycoprotein; G glycoprotein;VSV |
| Gene ID : | 1489834 |
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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