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Cat#: VRS-0124-YT5229
Lenti-SARS-CoV Spike Pseudovirus [β-galactosidase, HIV-1 Packaging System]
SARS-CoV spike pseudovirus is produced by cotransfecting HEK293 cells with the vector pHxLacZWP encoding -galactosidase (-Gal), pShCAG2-nSpike, and HIV packaging plasmid pCMVR8.2. Pseudovirus-containing supernatant was harvested 48 h following transfection. The supernatant was cleared of cell debris by centrifugation and filtered through a 0.45-μm-pore-size filter. The produced pseudoviruses carry SARS-CoV spike protein, which is a powerful tool for SARS-CoV vaccine and entry inhibitor research.
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- Product Summary
- Packaging, Storage & Formulations
- Target Details
Product Summary
| Type : | Pseudovirus |
| Virus Name : | Severe Acute Respiratory Syndrome Coronavirus |
| Virus Packaging Protein : | Spike |
| Packaging System : | HIV-1 Packaging System |
| Packaging Cell : | HEK293 |
| Reporter Gene : | β-Galactosidase |
| Titer : | >1x 108 virus copies/mL |
| Application Notes : | The pseudovirus can be usd for the following applications: 1. Antibody neutralization assay, Serum neutralization assay. 2. As virus standard alternative in diagnostic assay or others. 3. Drug screening including vaccine development, viral entry inhibitor development. 4. As a safe virology tool to discover the virus entry mechanisms. |
Packaging, Storage & Formulations
| Form : | Liquid |
| Storage : | Store at -80°C and stable for 6 months from the date of shipment, avoiding freeze/thaw cycles. |
| Shipping : | Dry ice |
| Note : | The pseudovirus can be used under BSL-2 conditions. Please reach out to our scientists for detailed instructions or guidelines of the pseudovirus. |
Target Details
| Introduction : | Severe acute respiratory syndrome (SARS) is a viral respiratory disease of zoonotic origin caused by SARS-CoV or SARS-CoV-1 infections. SARS-CoV is an enveloped, positive-sense, single-stranded RNA virus. The virus enters the host cell by the binding to the human angiotensin-converting enzyme 2 (ACE2) receptor. |
| Alternative Names : | SARS-CoV, SARS, S, Spike, Pseudovirus, Spike Pseudovirus, S Pseudovirus, SARS-CoV Spike Pseudovirus, SARS-CoV S Pseudovirus, SARS Spike Pseudovirus, SARS S Pseudovirus |
| Gene ID : | 1489668 |
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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