Lenti-RV GP Pseudovirus [Luciferase, HIV-1 Packaging System]

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Cat#: VRS-0124-YT5213

Lenti-RV GP Pseudovirus [Luciferase, HIV-1 Packaging System]

Lenti-RV GP Pseudovirus is produced by cotransfecting 293T cells with the plasmid enconding RV GP, the HIV type 1 (HIV-1) gag-pol construct pCMV-Δ8.91, and the firefly luciferase reporter construct pCSFLW. Pseudovirus-containing supernatant was harvested 48 h following transfection. The supernatant was cleared of cell debris by centrifugation and filtered through a 0.45-μm-pore-size filter. The produced pseudoviruses carrying RV GP is a powerful tool for studying mechanism of virus entry, antiviral mechanism, neutralization antibody assay, and screening antivirus inhibitor.

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  • Product Summary
  • Packaging, Storage & Formulations
  • Target Details

Product Summary

Type : Pseudovirus
Virus Name : Rabies Virus
Virus Packaging Protein : GP
Packaging System : HIV-1 Packaging System
Packaging Cell : 293T
Reporter Gene : Luciferase
Titer : >1x 108 virus copies/mL
Application Notes : The pseudovirus can be usd for the following applications:
1. Antibody neutralization assay, Serum neutralization assay.
2. As virus standard alternative in diagnostic assay or others.
3. Drug screening including vaccine development, viral entry inhibitor development.
4. As a safe virology tool to discover the virus entry mechanisms.

Packaging, Storage & Formulations

Form : Liquid
Storage : Store at -80°C and stable for 6 months from the date of shipment, avoiding freeze/thaw cycles.
Shipping : Dry ice
Note : The pseudovirus can be used under BSL-2 conditions. Please reach out to our scientists for detailed instructions or guidelines of the pseudovirus.

Target Details

Introduction : Rabies virus belongs to the order Mononegavirales, viruses with a nonsegmented, negative-stranded RNA genomes. Within this group, viruses with a distinct "bullet" shape are classified in the Rhabdoviridae family, which includes at least three genera of animal viruses, Lyssavirus, Ephemerovirus, and Vesiculovirus. Rhabdoviruses are approximately 180 nm long and 75 nm wide. The rabies genome encodes five proteins: nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G) and polymerase (L). All rhabdoviruses have two major structural components: a helical ribonucleoprotein core (RNP) and a surrounding envelope.
Alternative Names : Rabies virus glycoprotein; RV; RABVgp4; RV GP Pseudovirus
Gene ID : 1489856

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.


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