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Cat#: VRS-0124-YT5271
Lenti-NiV G/F Pseudovirus [GFP, HIV-1 Packaging System]
NiV G/F Pseudovirus is produced by cotransfecting 293T cells with NiV F and G proteins, pCSCGW (pCSC-SP-PW-GFP), pMDL, and pRSV-REV. Pseudovirus-containing supernatant was harvested 48 h following transfection. The supernatant was layered over a 20% sucrose cushion and concentrated by centrifugation at 28,000 rpm at 4°C for 2 h. The viral pellet was resuspended in phosphate-buffered saline (PBS) and filtered with a 0.45-μm-pore-size 0.45 μm filter).
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- Product Summary
- Packaging, Storage & Formulations
- Target Details
Product Summary
| Type : | Pseudovirus |
| Virus Name : | Nipah Virus |
| Virus Packaging Protein : | G/F |
| Packaging System : | HIV-1 Packaging System |
| Packaging Cell : | 293T |
| Reporter Gene : | GFP |
| Titer : | >1x 108 virus copies/mL |
| Application Notes : | The pseudovirus can be usd for the following applications: 1. Antibody neutralization assay, Serum neutralization assay. 2. As virus standard alternative in diagnostic assay or others. 3. Drug screening including vaccine development, viral entry inhibitor development. 4. As a safe virology tool to discover the virus entry mechanisms. |
Packaging, Storage & Formulations
| Form : | Liquid |
| Storage : | Store at -80°C and stable for 6 months from the date of shipment, avoiding freeze/thaw cycles. |
| Shipping : | Dry ice |
| Note : | The pseudovirus can be used under BSL-2 conditions. Please reach out to our scientists for detailed instructions or guidelines of the pseudovirus. |
Target Details
| Introduction : | Nipah virus (NiV) is a deadly pathogen, which is transmitted by bodily fluids from pteropid fruit bats and livestock to humans. It is a nonsegmented, single-stranded, negative-sense RNA virus belonging to the genus Henipavirus, family Paramyxoviridae. The NiV envelope contains two surface glycoproteins required for cellular entry: the attachment protein (NiV-G) and fusion protein (NiV-F). Niv-G allows for attachment of the virion to cellular receptors, whereas NiV-F mediates the direct union of the virus and cellular membranes. |
| Alternative Names : | NiV G; GP; F; G/F; NiV; NiV G/F Pseudovirus |
| Gene ID : | 920955;920954 |
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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