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Cat#: VRS-0124-YT5281
Lenti-JEV PrM/E Pseudovirus [Luciferase, VSV Packaging System]
The Huh7 cells expressing PrM and E proteins of JEV were infected with VSVΔG*/Luc-*G which encodes the luciferase gene. After 24 h the infected cell supernatants were collected and the pseudotyped viruses were purified by centrifugation through sucrose density gradients. The produced pseudoviruses carry JEV PrM and E proteins, which is a powerful tool for vaccine development and entry inhibitor research.
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- Product Summary
- Packaging, Storage & Formulations
- Target Details
Product Summary
| Type : | Pseudovirus |
| Virus Name : | Japanese Encephalitis Virus |
| Virus Packaging Protein : | PrM and E proteins |
| Packaging System : | VSV Packaging System |
| Packaging Cell : | Huh7 cells |
| Reporter Gene : | Luciferase |
| Titer : | >1x 108 virus copies/mL |
| Application Notes : | The pseudovirus can be usd for the following applications: 1. Antibody neutralization assay, Serum neutralization assay. 2. As virus standard alternative in diagnostic assay or others. 3. Drug screening including vaccine development, viral entry inhibitor development. 4. As a safe virology tool to discover the virus entry mechanisms. |
Packaging, Storage & Formulations
| Form : | Liquid |
| Storage : | Store at -80°C and stable for 6 months from the date of shipment, avoiding freeze/thaw cycles. |
| Shipping : | Dry ice |
| Note : | The pseudovirus can be used under BSL-2 conditions. Please reach out to our scientists for detailed instructions or guidelines of the pseudovirus. |
Target Details
| Introduction : | Japanese encephalitis virus (JEV) is a small, enveloped virus belonging to the family Flaviviridae and the genus Flavivirus. JEV is the most common agent of viral encephalitis, causing approximately 50,000 cases annually. JEV has a single-stranded positive-sense RNA genome of approximately 11 kb, encoding a single large polyprotein, which is cleaved by the host- and virus-encoded proteases into three structural proteins, capsid (C), premembrane (PrM), and envelope (E), and seven nonstructural proteins. The structural proteins are components of viral particles, and the E protein is suggested to interact with a cell surface receptor molecule. |
| Alternative Names : | JEV PrM/E Pseudovirus; PrM/E; Premembrane; Envelope; |
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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