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Cat#: VRS-0124-YT5220
Lenti-HIV-1 gp140 Pseudovirus [GFP, MLV Packaging System]
HIV-1 gp140 pseudovirus is produced by transfecting the cell lines TELCeB6 carrying a β-galactosidase reporter gene with HIV-1 gp140 expression plasmids. Pseudovirus-containing supernatant was harvested 48 h following transfection. The supernatant was cleared of cell debris by centrifugation and filtered through a 0.45-μm-pore-size filter. The produced pseudoviruses carry HIV-1 gp140 proteins, which is a powerful tool for HIV-1 vaccine and entry inhibitor research.
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- Product Summary
- Packaging, Storage & Formulations
- Target Details
Product Summary
| Type : | Pseudovirus |
| Virus Name : | Human Immunodeficiency Virus 1 |
| Virus Packaging Protein : | gp160 |
| Packaging System : | MLV Packaging System |
| Packaging Cell : | TELCeB6 cells |
| Reporter Gene : | β-Galactosidase |
| Titer : | >1x 108 virus copies/mL |
| Application Notes : | The pseudovirus can be usd for the following applications: 1. Antibody neutralization assay, Serum neutralization assay. 2. As virus standard alternative in diagnostic assay or others. 3. Drug screening including vaccine development, viral entry inhibitor development. 4. As a safe virology tool to discover the virus entry mechanisms. |
Packaging, Storage & Formulations
| Form : | Liquid |
| Storage : | Store at -80°C and stable for 6 months from the date of shipment, avoiding freeze/thaw cycles. |
| Shipping : | Dry ice |
| Note : | The pseudovirus can be used under BSL-2 conditions. Please reach out to our scientists for detailed instructions or guidelines of the pseudovirus. |
Target Details
| Introduction : | Human immunodeficiency virus (HIV) can be divided into two major types, HIV-1 and HIV-2. HIV-1 is the most common and pathogenic strain of the virus. The HIV-1 env gene encodes the gp160 glycoprotein, which is subsequently cleaved into the envelope proteins gp120 and gp41. gp120 is the viral surface protein that mediates attachment of the virus to target cells via binding to the CD4 receptor and a co-receptor such as CCR5. gp41 is a transmembrane protein that mediates fusion of the viral and cellular lipid membranes once gp120 has bound its cognate receptor and co-receptor. |
| Alternative Names : | HIV; HIV-1; AIDS; gp140; Env polyprotein; Pseudovirus; Envelope glycoprotein gp140; Envelope surface glycoprotein gp140; |
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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