The porcine epidemic diarrhea virus (PEDV) has emerged as one of the most lethal pathogens infecting pig herds, causing highly contagious intestinal infectious diseases characterized by severe enteritis, diarrhea, vomiting, and dehydration. Although PEDV was discovered in England in 1971, the etiological agent (CV777) was not identified until 1978, with the full-length genome only being determined in 2001. PEDV has now spread throughout the world.
PEDV is a positive-sense RNA coronavirus with a single strand that belongs to the genus Alphacoronavirus in the family Coronaviridae and the order Nidovirales. PEDV is enveloped, pleomorphic, and 95-190 nm in diameter, including projections that are about 18 nm long. The viral genome is about 28 kb long, with a 5' terminal cap, a 3' poly (A) tail, and seven open reading frames (ORFs), including ORF1a, ORF1b, spike (S), ORF3, envelope (E), membrane (M), and nucleocapsid (N) genes. ORFs 1a and 1b encode non-structural viral proteins (nsps). The ORF1a and ORF1b N termini encode two large replicase polyprotein precursors (pp1a and pp1ab), which can be cleaved into nsp1-16. These proteins are required for viral replication as well as anti-host immunity. The S protein is required for virus invasion of host cells and can stimulate the production of anti-PEDV specific antibodies. ORF3 encodes an accessory protein thought to be involved in virulence.
Fig.2 PEDV structure and its genome. (Li, et al., 2020)
PEDV is genetically classified into two groups: G1 (classical) and G2 (field epidemic or pandemic), with five subgenotypes in between (G1a, G1b, G2a, G2b, and G2c). The highly virulent PEDV-G2 virus emerged in 2010 and has caused massive economic losses to the global pork industry. In some Asian countries, PEDV-G1 vaccines are widely used. Live-attenuated or inactivated vaccines derived from PEDV-G1 strains such as CV777, DR13, P5-V, and SM98-1 are examples of vaccines. However, their efficacy against the newly emerged highly virulent PEDV-G2 strains was minimal, most likely due to antigenic differences between the S proteins of the G1 and G2 strains.
Creative Biolabs is the leading antibody supplier to customers worldwide for veterinary immune system research. PEDV antibodies are available in a variety of applications, species, and conjugated and unconjugated forms. These antibodies have been rigorously tested to ensure their purity and quality in order to expedite your research process. We also offer customized antibody development services, such as antibody discovery, engineering, and customized services. Please contact us if you require any additional information.