Infectious virus can interact with specific receptor, resulting virus entry into host cells. For example, SARS-CoV-2 consists of four structural proteins, including S (spike), E (envelope), M (membrane), and N (nucleocapsid) proteins. S, M and E proteins create the viral envelope together, and N protein holds the SARS-CoV-2 RNA genome. As a key component, spike (S) was identified as the protein responsible for virus entry into human cells. In addition, many articles propose and validate that the angiotensin converting enzyme 2 (ACE2) might be the SARS-CoV-2 infection receptor, and spike/ACE2 interaction might be the mechanism of host cell entry. To facilitate the virus in vitro assays, pseudohost is developed which is a stable cell line expressing the specific receptor, such as human angiotensin-converting enzyme 2 (hACE2) etc. Creative Biolabs developed hACE2 overexpressed 293T and CHO stable cell lines to advance your COVID-19 researches. Together with our pseudovirus, pseudohost can be used in the following applications.
- Pseudovirus-based inhibition assay for the detection of neutralizaitn antibodies.
- Pseudovirus-based inhibition assay for the detection of anti-virus entry inhibitors.
- Evaluating the vaccine candidate-induced neutralizing antibodies against the highly pathogenic virus.
- Virus entry mechanism discovery.
Fig.1 Structural and functional basis of SARS-CoV-2 entry by using human ACE2. (Wang, 2020)
Reference
- Wang, Qihui, et al. "Structural and functional basis of SARS-CoV-2 entry by using human ACE2." Cell (2020).