Documents
Cat#: VRS-1223-YT115
ViroAb™ Mouse Anti-Bovine Papillomavirus E2 Monoclonal Antibody (XW0028)
This Mouse Monoclonal antibody is specific for E2 protein of Bovine Papillomavirus. The antibody can be used for applications: WB, IF. The antibody is for research use only and is not approved for use in humans or in clinical diagnosis.
Made to Order Requirements
Your choose infomation:
CLEAR(Note: Please select the requirements to fit you project. If you have any other requirements, please specify. If no specific requirements, you don't need to select any button.)
- Target Published Figures
-
-
Immunofluorescent staining images of bovine enteroids (a-c) mock-infected and (d) infected with bovine coronavirus (BCoV) at 72 h post inoculation.
(a) F-actin expressing brush border on the luminal surface (Phalloidin, green); (b) stained for enterocytes (sucrase isomaltase, green); (c) stained for enteroendocrine cells (chromogranin A, red); and (d) stained for BCoV S-protein (green) and enteroendocrine cells (chromogranin A, red). Nuclei were counter-stained with Hoechst (blue). Magnification = 63×; Scale bar = 50 µm.
- Product Overview
- Product Properties
- Packaging, Storage & Formulations
- Applications
Product Overview
| Target : | E2 |
| Clone : | XW0028 |
| Host Species : | Mouse |
| Antibody Isotype : | IgG |
| Species Reactivity : | Bovine Papillomavirus |
Product Properties
| Immunogen : | Recombinant full length Bovine Papillomavirus E2-protein expressed in E Coli |
| Epitope : | Amino acids 208-218 (RPDGVWVASEG) |
| Purification : | Protein A affinity chromatography |
Packaging, Storage & Formulations
| Form : | Liquid |
| Formulation : | PBS, 50% Glycerol |
Applications
| Application : | WB; IF |
| Application Notes : | IF: 1/1000 WB: 1/1000 - 1/5000 The optimal working dilutions should be determined by the end user. |
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Shakya, Ruchika, et al. "Bovine Enteroids as an In Vitro Model for Infection with Bovine Coronavirus." Viruses 15.3 (2023): 635.
Virology-
(Verified Customer)
I purchased this antibody to test the bovine corona virus in fecal samples collected from infected calves. Hope it will work well.
Hi team,
I am interested in your product "VRSX-0823-YT122". Could you tell me what kind of samples this antibody could detect?
All the best
Taisei
Thank you for contacting us. This antibody product could detect the bovine corona virus generally in plasma, serum, and fecal samples of infected animals. Please don't hesitate to contact us if you need any further advice or information on other aspects.
Target Research Highlights
Bovine coronavirus (BCoV) is one of the major viral pathogens of cattle, responsible for economic losses and causing a substantial impact on animal welfare. 3D enteroids are a good model to investigate host-pathogen interactions. The 3D enteroids were successfully established from bovine ileum and permissive to BCoV, with a seven-fold increase in viral RNA after 72 h. Immunostaining of differentiation markers showed a mixed population of differentiated cells. This study shows that the bovine enteroids had a differentiated cell population and were permissive to BCoV.
References
Shakya, Ruchika, et al. "Bovine Enteroids as an In Vitro Model for Infection with Bovine Coronavirus." Viruses 15.3 (2023): 635.
pepFastX™ Platform
If no antibody is available for your research, we recommend our novel pepFastX™ antibody discovery platform. The mAb products can be delivered within 2-3 months.
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support