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Cat#: VRH-22410F
pepFastX™ Mouse Anti-VACV WR L1R (AVVDNKLKI) Monoclonal Antibody (22410YF)
This mouse monoclonal antibody 22410YF is generated from premade hybridoma library, and specific for Vaccinia virus WR L1R (AA 120-128): AVVDNKLKI. For any additional requirments, please specify and submit an inquiry for assistance.
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- Antibody Testing Data
- Target Published Figures
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Mouse Anti-VACV WR L1R (AVVDNKLKI) Monoclonal Antibody (22410YF) [Product # VRH-22410F] in ELISA
ELISA analysis of Mouse Anti-VACV WR L1R (AVVDNKLKI) Monoclonal Antibody (22410YF) [Product # VRH-22410F] at different dilutions. The secondary antibody used was HRP-conjugated Goat Anti-Mouse IgG (H+L) at 1:4000 dilution, 100 μL.
Antigen: Peptide AVVDNKLKI, 100 ng
Antibody Dilutions: 1:2.5K/5K/10K/20K/40K/80K/160K/320K/640K/1280K -
Mouse Anti-VACV WR L1R (AVVDNKLKI) Monoclonal Antibody (22410YF) [Product # VRH-22410F] in SDS-PAGE
SDS-PAGE analysis of Mouse Anti-VACV WR L1R (AVVDNKLKI) Monoclonal Antibody (22410YF) [Product # VRH-22410F] (lane 1) at reducing condition. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa.
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Anti-VACV L1R Monoclonal Antibody (Mab-10F5) in Immunoprecipitation
COS cell monolayers were transfected with VACV L1R construct and negative control construct (+ or -). The cell samples were immunoprecipitated with the anti-VACV L1R monoclonal antibody (Mab-10F5). Then the samples were boiled and analyzed by SDS-PAGE in reducing and nonreducing conditions. The markers in kDa are shown at lane MW. -
Neutralizing Antibody Responses Elicited by Vaccination with pWRG/L1R in Mice
Mean plaque-reduction neutralization tests (PRNT) values of different vaccinations are shown in the figure (vaccination with pWRG/L1R, pWRG/A33R, pWRG/A33R + pWRG/L1R, Mab-7D11, VACV HMAF, negative control). Neutralizing antibody response was elicited by vaccination with pWRG/L1R. -
L1R Transcript Expression by Western Blotting Validation in Replication-permissive and Non-permissive Cell Lines
3 different cell lines including CEFs, Vero and MRC-5 were infected with FP(L1R). WB analysis of the cell samples were performed using specific antibodies in replication-permissive (lanes B) and non-permissive cell lines (lanes A). -
L1R Protein Localisation by Immunofluorescence
3 different cell lines including CEFs, Vero and MRC-5 were infected with FP(L1R) (1a-1c). Immunofluorescence analysis of the cell samples were performed, and the cytoplasmic immunofluorescence was diffuse.
- Product Overview
- Product Properties
- Applications
Product Overview
| Target : | L1R |
| Specificity : | VACV WR |
| Clone : | 22410YF |
| Host Species : | Mouse |
| Antibody Isotype : | IgG |
| Species Reactivity : | Vaccinia virus |
Product Properties
| Immunogen : | Synthetic peptide corresponding to amino acid sequence: AVVDNKLKI. |
| Epitope : | AVVDNKLKI |
| Purity : | >95% |
| Purification : | Protein A or G affinity chromatography |
| Concentration : | Lot specific |
Applications
| Application : | ELISA |
| Application Notes : | The antibody is screened by ELISA using the synthetic peptide that corresponds to amino acids sequence (AVVDNKLKI) as the capture antigen. Additional applications have not been validated. It is recommended that the end users validate other applications and determine optimal concentrations/dilution under their experimental conditions. |
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
DNA vaccination with vaccinia virus L1R and A33R genes protects mice against a lethal poxvirus challenge.
VirologyL1R, A27L, A33R and B5R vaccinia virus genes expressed by fowlpox recombinants as putative novel orthopoxvirus vaccines.
Virology-
(Verified Customer)
I ordered the Mouse Anti-VACV WR L1R (AVVDNKLKI) Monoclonal Antibody (22410YF), and will test it in Western Blot analysis with L1R antigen. Hope it will work well.
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(Verified Customer)
The purity of this VACV WR L1R antibody is verified to be>95% as determined by SDS-PAGE gel.
I would like to know the peptide seq to which this antibody recognizes in the VACV WR L1R protein.
The antigen of this antibody used for screening is peptide - AVVDNKLKI.
Dear sir,
We're interested in VRH-22410F. Please help us confirm if this antibody can be used for neutralization assays?
Regards,
Wang, Yiwei
Thank you for contacting us. We have not tested the neutralization capability of this antibody. I am sorry for not being able to provide you any useful information this time. Please don't hesitate to contact us if you need any further advice or information in other aspects.
Target Research Highlights
The author constructed a recombinant vaccinia virus (VACV) (vL1Ri) which can express L1, and demonstrated that L1 protein of VACV is essential for virus assembly, cell entry and membrane fusion. Moreover, L1 was proved to be interacted with the entry/fusion complex (EFC) as an another component.
References
Bisht, Himani, Andrea S. Weisberg, and Bernard Moss. "Vaccinia virus L1 protein is required for cell entry and membrane fusion." Journal of virology 82.17 (2008): 8687-8694.
Target Research Highlights
This article analyzed the roles of L1 and A28 of vaccinia virus (VACV) for cellular entry. The results indicated that a soluble, truncated form of L1 serves as receptor binding proteins (RBP) independently of glycosaminoglycans (GAGs).
References
Foo, Chwan Hong, et al. "Vaccinia virus L1 binds to cell surfaces and blocks virus entry independently of glycosaminoglycans." Virology 385.2 (2009): 368-382.
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