Fig.1 A schematic diagram of the ZIKV polyprotein (top panel) and topological arrangement of individual viral proteins (bottom panel).
Fig.2 Life cycle of the Flaviviridae.
Zika virus (ZIKV) belongs to the larger group of arboviruses (arthropod borne viruses). The Aedes genus of mosquitoes is the primary route by which ZIKV is transmitted. Of all the known cases of ZIKV infections, 80% are asymptomatic. The symptoms and signs of patients affected by ZIKV are similar to Dengue virus (DENV) and other viral diseases. The patient may present with fever, body aches, joint pains, fatigue, malaise, and conjunctivitis. Two main lineages have been identified so far from entire genome sequencing namely, the African and the Asian lineages. Further divided into two groups, the African lineage is composed of clusters Uganda and Nigeria. Study demonstrated that single serine to asparagine substitution (S139N) in the viral polyprotein can lead to enhanced infectivity of human and mouse neural progenitor cells (NPCs) by ZIKV. It was also suggested that this functional adaptation can lead to microcephaly in the mouse fetus, and higher mortality in neonatal mice.
ZIKV is a positive-sense RNA that is single-stranded. Only a single polyprotein is encoded by its 10.7 kb genome. It is further cleaved into 10 proteins, three of which are structural and seven of which are non-structural (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5).
What Creative Biolabs offers?
Creative Biolabs has become a leader of recombinant antibody (rAb) discovery, engineering and manufacturing, providing high quality services to customers in academic and industry fields all over the world. Now we focus on antibody discovery services for ZIKV research. Learn more about our ViroAntibody Discovery Services. Creative Biolabs now provides a variety of ZIKV antibodies targeting the above proteins to meet our clients’ requirements. Customized antibody development services and antibody related virology assays are also available for you. Please submit your specific antibody inquiry to us. We also provide a full comprehensive suite of secondary antibodies and isotype controls to meet your needs. For any questions, please feel free to contact us for more information.