Fig.1 Virion structure and genome organization of MARV. (Abir, et al., 2022)
Marburg virus (MARV) is a member of the Filoviridae family with a 19.1 kb non-segmented, single-stranded, negative-sense RNA genome. It is a close relative of the Ebola virus (EBOV), but they function very differently. Because Marburg had the most cases, the virus was named after the city. MARV disease is a rare and highly virulent disease that causes hemorrhagic fever and has a fatality rate of up to 88%. The disease is transmitted to humans by fruit bats and spreads between humans via bodily fluid transmission.
MARVs are about 80 nm in diameter and 800 to 1100 nm in length, but they frequently take on longer and even branching forms. The virus is a single strand of non-segmented RNA with seven monocistronic genes (each mRNA only codes for one protein) arranged in a linear fashion. Nucleoprotein (NP), polymerase cofactor (VP35), matrix protein (VP40), glycoprotein (GP), replication-transcription protein (VP30), minor matrix protein (VP24), and RNA-dependent RNA polymerase (L) are the seven structural proteins.
The Marburg virus enters the body in three stages: cellular attachment, endocytosis, and fusion. The nucleocapsid is released into the cytoplasm when the virus membrane fuses with the vesicle membrane. Massive amounts of nucleocapsids accumulate intracellularly during infection, forming intracytoplasmic inclusion bodies that serve as sites for MARV transcription, replication, and nucleocapsid assembly. Mature nucleocapsids are transported to the plasma membrane and MARV buds for envelopment and release. MARV infiltrates the host and spreads to the lymphatic and vascular systems. Infection of endothelial and parenchymal cells in many organs causes additional tissue damage. Many organs are necrotic as a result of MARV, including the liver, spleen, kidneys, gastrointestinal tract, and endocardium.
Fig.2 MARV entry, viral dissemination, and cellular tropism. (Abir, et al., 2022)
There are virological, serological, and molecular diagnostic methods for MARV detection, such as virus isolation, ELISA, RT-PCR, electron microscopy (EM), and immunohistochemistry.
Creative Biolabs, an antibody expert, now offers a variety of anti-MARV antibody products that target the different viral proteins. We also offer customized antibody development services against various MARV targets. If you have any questions about our anti-MARV antibody products or services, please contact us.