Retro-SARS-CoV-2 ORF1 a/b & E & N Pseudovirus (CAT#: PSV-003F)

SARS-CoV-2 ORF1 a/b & E & N pseudovirus is generated by co-transfection 293T cells with retroviral vector and plasmid encoding partial ORF1 a/b, E and N genes of SARS-CoV-2. The supernatants were harvested at 64 hours post transfection. The supernatants are centrifuged to remove cell debris and passed through filter to get pseudovirus. The pseudovirus consists of retrovirus envelope and SARS-CoV-2 ORF1 a/b & E & N. They can be used as a positive control for viral RNA extraction experiement and qPCR testing. Pseudoviruses are useful virological tools because of their safety and versatility.

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Product Details
Virus Packaging ProteinORF1 a/b & E & N
Packaging SystemRetrovirus
Packaging Cell293T
Schematic diagram
Application NotesThe pseudovirus can be usd for the following applications:
1. As a positive control for viral RNA extraction experiement and qPCR testing.
2. As virus standard alternative in diagnostic assay or others.
Titer>1^8 virus copies/mL
StorageStore at -80°C and stable for 6 months from the date of shipment, avoiding freeze/thaw cycles.
NoteThe pseudovirus can be used under BSL-2 conditions. Please reach out to our scientists for detailed instructions or guidelines of the pseudovirus.
Pseudovirus Details
Virus FamilyCoronaviridae
Virus SpeciesSARS-CoV-2
Virus ClassificationPositive-sense RNA Virus
Related DiseaseCOVID-19
IntroductionAn outbreak of a novel coronavirus disease (COVID-19; previously known as 2019-nCoV) was reported since last December, 2019. Now COVID-19 is an emerging, rapidly evolving situation, and has become a pandemic.SARS-CoV-2 consists of four structural proteins, including S (spike), E (envelope), M (membrane), and N (nucleocapsid) proteins. S, M and E proteins create the viral envelope together, and N protein holds the SARS-CoV-2 RNA genome.
Alternative NamesSARS-CoV-2, 2019nCoV, COVID-19, S, Spike, Pseudovirus, Spike Pseudovirus, S Pseudovirus, SARS-CoV-2 Spike Pseudovirus, SARS-CoV-2 S Pseudovirus, COVID-19 Spike Pseudovirus, COVID-19 S Pseudovirus
All products and services are intended for Research Use Only, and NOT to be used in diagnostic or therapeutic procedures.

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