Certificate of Analysis Lookup
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HIV-1 gp160 pseudovirus is produced by cotransfecting 293T/17 cells with a plasmid inserted an HIV-1 env gene derived from Bx08.16 isolate and an env-deficient HIV-1 backbone plasmid using Lipofectamine 2000 (Invitrogen). Pseudovirus-containing supernatant was harvested 24 h following transfection. The supernatant was cleared of cell debris by centrifugation and filtered through a 0.45-μm-pore-size filter. The produced pseudoviruses carry HIV-1 gp160 proteins, which is a powerful tool for HIV-1 vaccine and entry inhibitor research.
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|Virus Packaging Protein||gp160|
|Packaging Cell||293T/17 cells|
|Application Notes||The pseudovirus can be usd for the following applications:|
1. Antibody neutralization assay, Serum neutralization assay.
2. As virus standard alternative in diagnostic assay or others.
3. Drug screening including vaccine development, viral entry inhibitor development.
4. As a safe virology tool to discover the virus entry mechanisms.
|Titer||>1^8 virus copies/mL|
|Storage||Store at -80°C and stable for 6 months from the date of shipment, avoiding freeze/thaw cycles.|
|Note||The pseudovirus can be used under BSL-2 conditions. Please reach out to our scientists for detailed instructions or guidelines of the pseudovirus.|
|Virus Species||HIV-1 Bx08.16 isolate|
|Virus Classification||Positive-sense RNA Virus|
|Introduction||Human immunodeficiency virus (HIV) can be divided into two major types, HIV-1 and HIV-2. HIV-1 is the most common and pathogenic strain of the virus. The HIV-1 env gene encodes the gp160 glycoprotein, which is subsequently cleaved into the envelope proteins gp120 and gp41. gp120 is the viral surface protein that mediates attachment of the virus to target cells via binding to the CD4 receptor and a co-receptor such as CCR5. gp41 is a transmembrane protein that mediates fusion of the viral and cellular lipid membranes once gp120 has bound its cognate receptor and co-receptor.|
|Alternative Names||HIV; HIV-1; AIDS; gp160; Env polyprotein; Pseudovirus; Envelope glycoprotein gp160; Envelope surface glycoprotein gp160;Envelope precursor|