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Lot Number
Rabbit Anti-Bovine respiratory syncytial virus (BRSV) F Polyclonal Antibody (WB, ELISA) (CAT#: VRX-0543J)
This Rabbit Polyclonal antibody is specific for F protein of Bovine respiratory syncytial virus (BRSV). The antibody can be used for applications: WB, ELISA. The antibody is for Respiratory tract infection disease research use only and is not approved for use in humans or in clinical diagnosis.
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| Product Summary | |
|---|---|
| Product Category | Primary antibody |
| Target | F |
| Host | Rabbit |
| Species Reactivity | Bovine respiratory syncytial virus (BRSV) |
| Clonality | Polyclonal |
| Application | WB, ELISA |
| Product Details | |
|---|---|
| Specificity | This antibody recognizes bovine residues 483-488 [FPSDEFC] of the 63kD RSV and BRSV Fusion glycoprotein. |
| Immunogen | Synthetic peptide, aa483-488 (FPSDEFC) of the 63kD RSV and BRSV Fusion glycoprotein. |
| Epitope | Bovine residues 483-488 [FPSDEFC] of the 63kD RSV |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Antigen Details | |
|---|---|
| Full Name | Fusion glycoprotein |
| Introduction | During virus entry, induces fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm. The fusogenic activity is inactive untill entry into host cell endosome, where a furin-like protease cleaves off a small peptide between F1 and F2. Interacts directly with heparan sulfate and may participate in virus attachment. Later in infection, proteins F expressed at the plasma membrane of infected cells can mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis. The fusion protein is also able to trigger p53-dependent apoptosis. |
| Gene ID | 37616284 |
| UniProt ID | P29791 |
| Alternative Names | Fusion glycoprotein F2, Fusion glycoprotein F1, F0 |
| Related Disease | Respiratory tract infection |
| Virus Details | |
|---|---|
| Virus Family | Paramyxoviridae |
| Virus Species | Bovine respiratory syncytial virus (BRSV) |
| Virus Classification | Negative-sense RNA Viruses |
| Packaging, Storage & Formulations | |
|---|---|
| Purification | Affinity chromatography purified |
| Storage | Store at 4°C for short term. Store at -20°C or -80°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Buffer | PBS (pH7.2) with 0.02% Sodium Azide and 40% Glycerol |
| Form | Liquid |
| Preservative | 0.02% Sodium Azide |
| Formulation | PBS (pH7.2) with 0.02% Sodium Azide and 40% Glycerol |
| Purity | >95% as determined by SDS-PAGE |
| Application Notes | |
|---|---|
| Application | WB, ELISA |
| Application Notes | WB: 1:250-1:500 The optimal working dilutions should be determined by the end user. |
All products and services are intended for Research Use Only, and NOT to be used in diagnostic or therapeutic procedures.
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Application
3D IHC3D Immunohistochemistry
ActivActivation Assay
AgonistAgonist
ALPHAAmplified Luminescent Proximity Homogeneous Assay
AntagonistAntagonist
ADCCAntibody-dependent Cellular Cytotoxicity Assay
ApopApoptosis Assay
BABioAssay
BIBioimaging
BlockBlocking
CACell-based Assay/Cellular Assay
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CDCComplement-dependent Cytotoxicity Assay
CostimCostimulation Assay
CytCytotoxicity Assay
DepDepletion Assay
DBDot Blot/Slot blot
ECLElectrochemiluminescence
EMElectron Microscopy
EMSAElectrophoretic Mobility Shift Assay
ELISAEnzyme-Linked Immunosorbent Assay
ELISpotEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FIAFluorescence Immunoassay
FRETFluorescence Resonance Energy Transfer Microscopy
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination Assay
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IEPImmunoelectrophoresis
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology-Resin Sections
IMImmunomicroscopy
IPImmunoprecipitation
IRMAImmunoradiometric Assay
ISHIn Situ Hybridization
InhibInhibition Assay
ICFCIntracellular Staining for Flow Cytometry
Live-cell imagingLive-cell Imaging Assay
CyTOFMass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MAMicroagglutination Assay
NeutNeutralization Assay
PAPeptide Array
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation Assay
SPRSurface Plasmon Resonance
TCAT-Cell Activation Assay
TRFTime-Resolved Fluorescence
TCTissue Culture
TBTurbidimetry
WBWestern Blot
HIHemagglutinin Inhibition
FLISAFluorescence-linked immunosorbent assay
CtrlControl
Dual ISH-IHCDual In Situ Hybridization-Immunohistochemistry
ICFCIntracellular Staining for Flow Cytometry
KDKnockdown Validated
BLIBio-Layer Interferometry
LFIALateral-Flow Immunochromatographic Assay
CryCrystallization
LFALateral flow assay
