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Cat#: VRS-1223-YT116
pepFastX™ Mouse Anti-Canine Circovirus (Strain UCD1-1698) Monoclonal Antibody (CF0904)
This mouse monoclonal antibody CF0904 is generated from premade mouse antibody library, and specific for Canine circovirus. The recognized peptide can be customized according to the specific virus complete sequence. For any additional requirments, please specify and submit an inquiry for assistance.
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- Target Published Figures
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Reactivity of E2-specific MAbs with the native E2 protein.
The mobility shift assay was carried out with 2 ng of bacterially expressed and purified E2 protein and 0.2 ng of radiolabelled E2 binding site for 15 min at room temperature. -
CHO4.15 cells constitutively expressing BPV-1 E1 and E2 proteins were electroporated with 100 ng of reporter plasmid pUCAlu and various concentrations of MAbs.
Cells were harvested 72 h after electroporation. Episomal DNA was digested with DpnI and linearizing enzyme HindIII and analyzed by Southern blotting. The replication signals of three independent experiments were quantified with a PhosphorImager, and signals from cells transfected with the origin-containing plasmid only were used as a control to normalize the results. Symbols: ●, MAb 3F12; ○, MAb 1E4; ▴, MAb 1H10; ■, MAb 5H4; □, nonspecific anti-β-galactosidase (β-gal) MAb.
- Product Overview
- Product Properties
- Applications
Product Overview
| Clone : | CF0904 |
| Host Species : | Mouse |
| Antibody Isotype : | IgG |
| Species Reactivity : | Canine circovirus |
Product Properties
| Purity : | >95% |
| Purification : | Protein A or G affinity chromatography |
| Concentration : | Lot specific |
Applications
| Application : | ELISA |
| Application Notes : | The antibody is screened by ELISA using the synthetic peptide that corresponds to a partial sequence of canine circovirus. Additional applications have not been validated. It is recommended that the end users validate other applications and determine optimal concentrations/dilution under their experimental conditions. |
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Kurg, Reet, et al. "Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication." Journal of virology 73.6 (1999): 4670-4677.
Virology-
(Verified Customer)
I bought this bovine papillomavirus E2 specific antibody for the specific detection of bovine papillomavirus by the western blot assay. The results of the experiment showed that the antibody had a good specificity.
Hi team,
I am interested in your product "VRSX-0823-YT125". Could you tell me the epitope of this anti-bovine Papillomavirus E2 antibody?
All the best
Brent
Thank you for contacting us. The epitope sequence of this product is RPDGVWVASEG. Please don't hesitate to contact us if you need any further advice or information on other aspects.
Target Research Highlights
The bovine papillomavirus type 1 (BPV-1) E2 protein is the master regulator of papillomavirus replication and transcription. Monoclonal antibodies (MAbs) against the BPV-1 E2 protein were used to probe the structure and function of the protein. Cotransfection of several MAbs with the BPV-1 minimal origin plasmid pUCAlu into CHO4.15 cells resulted in a dose-dependent inhibition of replication. MAbs directed against the hinge region sterically hinder the inter- or intramolecular interactions required for the replication activity of the E2 protein.
References
Kurg, Reet, et al. "Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication." Journal of virology 73.6 (1999): 4670-4677.
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