Human NPC1 Overexpressed CHO Cell Line Pseudohost

Datasheet

MSDS

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Lot Number

Product Details
Cell Type	CHO
Application Notes	The pseudohost can be usd for the following applications: 1. Antibody neutralization assay, Serum neutralization assay. 3. Drug screening including vaccine development, viral entry inhibitor development. 4. Virus entry mechanism discovery.
Related Disease	Hemorrhagic fever
Mycoplasma Testing	The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Neutralization Assay	Validated
Quality Control	Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.
Storage	Cells must be thawed immediately upon receipt and grown according to handling procedures to ensure cell viability and proper assay performance. DO NOT FREEZE the cells upon receipt as it may result in irreversible damage to the cell line. After recovery, the cells can be stored at -80°C or liquid nitrogen (-150°C to -190°C) for long term.
Note	We cannot guarantee cell viability if the cells are not thawed immediately upon receipt. Avoid multiple freeze/thaw cycles.

Product Details

Cell Type

CHO

Application Notes

The pseudohost can be usd for the following applications:
1. Antibody neutralization assay, Serum neutralization assay.
3. Drug screening including vaccine development, viral entry inhibitor development.
4. Virus entry mechanism discovery.

Related Disease

Hemorrhagic fever

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Neutralization Assay

Validated

Quality Control

Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.

Storage

Cells must be thawed immediately upon receipt and grown according to handling procedures to ensure cell viability and proper assay performance. DO NOT FREEZE the cells upon receipt as it may result in irreversible damage to the cell line. After recovery, the cells can be stored at -80°C or liquid nitrogen (-150°C to -190°C) for long term.

Note

We cannot guarantee cell viability if the cells are not thawed immediately upon receipt. Avoid multiple freeze/thaw cycles.

Receptor Details
Infection Receptor	NPC1
Introduction	NPC1 (Niemann-Pick C1) protein is a putative integral membrane protein containing sequence motifs consistent with a role in intracellular transport of cholesterol to post-lysosomal destinations. This protein transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolized and released as free cholesterol. The host-encoded NPC1 appears to be essential for entry of Ebola virions into the host cell and for its ultimate replication. When cells from NPC1 individuals lacking this transporter were exposed to Ebola virus in the laboratory, the cells survived and appeared impervious to the virus.
Gene ID	4864
Alternative Names	NPC; POGZ; SLC65A1

Receptor Details

Infection Receptor

NPC1

Introduction

NPC1 (Niemann-Pick C1) protein is a putative integral membrane protein containing sequence motifs consistent with a role in intracellular transport of cholesterol to post-lysosomal destinations. This protein transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolized and released as free cholesterol. The host-encoded NPC1 appears to be essential for entry of Ebola virions into the host cell and for its ultimate replication. When cells from NPC1 individuals lacking this transporter were exposed to Ebola virus in the laboratory, the cells survived and appeared impervious to the virus.

Gene ID

4864

Alternative Names

NPC; POGZ; SLC65A1

Human NPC1 Overexpressed CHO Cell Line Pseudohost

Human NPC1 Overexpressed CHO Cell Line Pseudohost (CAT#: PSH-004CQ)

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