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Human NPC1-overexpressed CHO pseudohost is produced by transfection CHO cells with a lentiviral vector encoding human NPC1 proteins. This stable cell line express human NPC1 proteins on the cell surface, and it can be used for drug/vaccine/viral inhibitor screening, neutralizing antibody screening and serum neutralization assay.
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|Application Notes||The pseudohost can be usd for the following applications:|
1. Antibody neutralization assay, Serum neutralization assay.
3. Drug screening including vaccine development, viral entry inhibitor development.
4. Virus entry mechanism discovery.
|Related Disease||Hemorrhagic fever|
|Mycoplasma Testing||The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.|
|Quality Control||Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.|
|Storage||Cells must be thawed immediately upon receipt and grown according to handling procedures to ensure cell viability and proper assay performance. DO NOT FREEZE the cells upon receipt as it may result in irreversible damage to the cell line. After recovery, the cells can be stored at -80°C or liquid nitrogen (-150°C to -190°C) for long term.|
|Note||We cannot guarantee cell viability if the cells are not thawed immediately upon receipt. Avoid multiple freeze/thaw cycles.|
|Introduction||NPC1 (Niemann-Pick C1) protein is a putative integral membrane protein containing sequence motifs consistent with a role in intracellular transport of cholesterol to post-lysosomal destinations. This protein transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolized and released as free cholesterol. The host-encoded NPC1 appears to be essential for entry of Ebola virions into the host cell and for its ultimate replication. When cells from NPC1 individuals lacking this transporter were exposed to Ebola virus in the laboratory, the cells survived and appeared impervious to the virus.|
|Alternative Names||NPC; POGZ; SLC65A1|