Mouse Anti-SARS-CoV / SARS-CoV-2 N Monoclonal Antibody (XF0017) (CAT#: VRX-0058F)

This Mouse Monoclonal antibody (XF0017) is specific for SARS-CoV / SARS-CoV-2 N protein. The antibody can be used for applications: WB, ICC/IF. The antibody is for Severe acute respiratory syndrome (SARS) disease research use only and is not approved for use in humans or in clinical diagnosis.

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Product Summary
Product CategoryPrimary Antibody
TargetN
HostMouse
Species ReactivitySARS-CoV, SARS-CoV-2
ClonalityMonoclonal
ApplicationWB, ICC, IF
Product Details
SpecificityThis antibody reacts with NP protein of SARS-CoV and SARS-CoV-2.
CloneXF0017
ImmunogenThe immunogen used to generate this antibody corresponds to SARS-CoV Nucleocapsid (121-422 a.a.).
IsotypeIgG1
ConjugationUnconjugated
Antigen Details
Full NameNucleocapsid Protein
IntroductionCoronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Gene ID43740575
Alternative NamesNucleocapsid protein, Nucleocapsid, Nucleoprotein, N, NP, Coronavirus NP, Coronavirus Nucleocapsid protein, Coronavirus Nucleocapsid, Coronavirus Nucleoprotein, Coronavirus N, SARS-CoV-2 NP, SARS-CoV-2 Nucleocapsid protein, SARS-CoV-2 Nucleocapsid, SARS-CoV-2 Nucleoprotein, SARS-CoV-2 N
Related DiseaseSevere acute respiratory syndrome (SARS)/COVID-19
Virus Details
Virus FamilyCoronaviridae
Virus SpeciesSARS-CoV/SARS-CoV-2
Virus ClassificationPositive-sense RNA Virus
Packaging, Storage & Formulations
PurificationProtein G purified
StorageStore at 4°C for short term (1-2 weeks). Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.
BufferPBS
FormLiquid
Purity˃ 95% as determined by SDS-PAGE
Application Notes
ApplicationWB, ICC, IF
Application NotesWB: 1:1000-1:10000
The optimal working dilutions should be determined by the end user.
All products and services are intended for Research Use Only, and NOT to be used in diagnostic or therapeutic procedures.
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3D IHC3D Immunohistochemistry
ActivActivation Assay
AgonistAgonist
ALPHAAmplified Luminescent Proximity Homogeneous Assay
AntagonistAntagonist
ADCCAntibody-dependent Cellular Cytotoxicity Assay
ApopApoptosis Assay
BABioAssay
BIBioimaging
BlockBlocking
CACell-based Assay/Cellular Assay
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CDCComplement-dependent Cytotoxicity Assay
CostimCostimulation Assay
CytCytotoxicity Assay
DepDepletion Assay
DBDot Blot/Slot blot
ECLElectrochemiluminescence
EMElectron Microscopy
EMSAElectrophoretic Mobility Shift Assay
ELISAEnzyme-Linked Immunosorbent Assay
ELISpotEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FIAFluorescence Immunoassay
FRETFluorescence Resonance Energy Transfer Microscopy
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination Assay
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IEPImmunoelectrophoresis
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology-Resin Sections
IMImmunomicroscopy
IPImmunoprecipitation
IRMAImmunoradiometric Assay
ISHIn Situ Hybridization
InhibInhibition Assay
ICFCIntracellular Staining for Flow Cytometry
Live-cell imagingLive-cell Imaging Assay
CyTOFMass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MAMicroagglutination Assay
NeutNeutralization Assay
PAPeptide Array
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation Assay
SPRSurface Plasmon Resonance
TCAT-Cell Activation Assay
TRFTime-Resolved Fluorescence
TCTissue Culture
TBTurbidimetry
WBWestern Blot
HIHemagglutinin Inhibition
FLISAFluorescence-linked immunosorbent assay
CtrlControl
Dual ISH-IHCDual In Situ Hybridization-Immunohistochemistry
ICFCIntracellular Staining for Flow Cytometry
KDKnockdown Validated
BLIBio-Layer Interferometry
LFIALateral-Flow Immunochromatographic Assay
CryCrystallization
LFALateral flow assay

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