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Lot Number
Mouse Anti-SARS-CoV / SARS-CoV-2 N Monoclonal Antibody (XF0017) (CAT#: VRX-0058F)
This Mouse Monoclonal antibody (XF0017) is specific for SARS-CoV / SARS-CoV-2 N protein. The antibody can be used for applications: WB, ICC/IF. The antibody is for Severe acute respiratory syndrome (SARS) disease research use only and is not approved for use in humans or in clinical diagnosis.
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| Product Summary | |
|---|---|
| Product Category | Primary Antibody |
| Target | N |
| Host | Mouse |
| Species Reactivity | SARS-CoV, SARS-CoV-2 |
| Clonality | Monoclonal |
| Application | WB, ICC, IF |
| Product Details | |
|---|---|
| Specificity | This antibody reacts with NP protein of SARS-CoV and SARS-CoV-2. |
| Clone | XF0017 |
| Immunogen | The immunogen used to generate this antibody corresponds to SARS-CoV Nucleocapsid (121-422 a.a.). |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Antigen Details | |
|---|---|
| Full Name | Nucleocapsid Protein |
| Introduction | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| Gene ID | 43740575 |
| Alternative Names | Nucleocapsid protein, Nucleocapsid, Nucleoprotein, N, NP, Coronavirus NP, Coronavirus Nucleocapsid protein, Coronavirus Nucleocapsid, Coronavirus Nucleoprotein, Coronavirus N, SARS-CoV-2 NP, SARS-CoV-2 Nucleocapsid protein, SARS-CoV-2 Nucleocapsid, SARS-CoV-2 Nucleoprotein, SARS-CoV-2 N |
| Related Disease | Severe acute respiratory syndrome (SARS)/COVID-19 |
| Virus Details | |
|---|---|
| Virus Family | Coronaviridae |
| Virus Species | SARS-CoV/SARS-CoV-2 |
| Virus Classification | Positive-sense RNA Virus |
| Packaging, Storage & Formulations | |
|---|---|
| Purification | Protein G purified |
| Storage | Store at 4°C for short term (1-2 weeks). Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Buffer | PBS |
| Form | Liquid |
| Purity | ˃ 95% as determined by SDS-PAGE |
| Application Notes | |
|---|---|
| Application | WB, ICC, IF |
| Application Notes | WB: 1:1000-1:10000 The optimal working dilutions should be determined by the end user. |
All products and services are intended for Research Use Only, and NOT to be used in diagnostic or therapeutic procedures.
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Application
3D IHC3D Immunohistochemistry
ActivActivation Assay
AgonistAgonist
ALPHAAmplified Luminescent Proximity Homogeneous Assay
AntagonistAntagonist
ADCCAntibody-dependent Cellular Cytotoxicity Assay
ApopApoptosis Assay
BABioAssay
BIBioimaging
BlockBlocking
CACell-based Assay/Cellular Assay
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CDCComplement-dependent Cytotoxicity Assay
CostimCostimulation Assay
CytCytotoxicity Assay
DepDepletion Assay
DBDot Blot/Slot blot
ECLElectrochemiluminescence
EMElectron Microscopy
EMSAElectrophoretic Mobility Shift Assay
ELISAEnzyme-Linked Immunosorbent Assay
ELISpotEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FIAFluorescence Immunoassay
FRETFluorescence Resonance Energy Transfer Microscopy
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination Assay
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IEPImmunoelectrophoresis
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology-Resin Sections
IMImmunomicroscopy
IPImmunoprecipitation
IRMAImmunoradiometric Assay
ISHIn Situ Hybridization
InhibInhibition Assay
ICFCIntracellular Staining for Flow Cytometry
Live-cell imagingLive-cell Imaging Assay
CyTOFMass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MAMicroagglutination Assay
NeutNeutralization Assay
PAPeptide Array
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation Assay
SPRSurface Plasmon Resonance
TCAT-Cell Activation Assay
TRFTime-Resolved Fluorescence
TCTissue Culture
TBTurbidimetry
WBWestern Blot
HIHemagglutinin Inhibition
FLISAFluorescence-linked immunosorbent assay
CtrlControl
Dual ISH-IHCDual In Situ Hybridization-Immunohistochemistry
ICFCIntracellular Staining for Flow Cytometry
KDKnockdown Validated
BLIBio-Layer Interferometry
LFIALateral-Flow Immunochromatographic Assay
CryCrystallization
LFALateral flow assay
