Mouse Anti-MERS-CoV Spike Monoclonal Antibody (XS-0234), IgG2b(Fc Engineered), Kappa

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Lot Number

Product Summary
Product Category	Primary Antibody
Target	S
Host	Mouse
Species Reactivity	; MERS-CoV;
Clonality	Monoclonal
Application	; Block; Neut;

Product Summary

Product Category

Primary Antibody

Target

Host

Mouse

Species Reactivity

; MERS-CoV;

Clonality

Monoclonal

Application

; Block; Neut;

Product Details
Specificity	This antibody specifcally binds the amino acids 349-590 in the S1 domain of the MERS-CoV.
Clone	XS-0234
Immunogen	The original antibody was generated by isolating neutralizing antibodies from a non-immune human Ab-phage library using a novel panning strategy.
Isotype	IgG2b(Fc Engineered), Kappa
Conjugation	Unconjugated

Product Details

Specificity

This antibody specifcally binds the amino acids 349-590 in the S1 domain of the MERS-CoV.

Clone

XS-0234

Immunogen

The original antibody was generated by isolating neutralizing antibodies from a non-immune human Ab-phage library using a novel panning strategy.

Isotype

IgG2b(Fc Engineered), Kappa

Conjugation

Unconjugated

Antigen Details
Full Name	Spike glycoprotein
Introduction	The MERS-CoV spike (S) protein is a type I transmembrane glycoprotein which contains 1353 amino acids and can be cleaved into two subunit S1 and S2. It forms large protruding spikes on the surface of the virus. The S1 subunit which contains RBD is responsible for binding to Dipeptidyl peptidase 4, which serves as the host cell receptor of MERS-CoV and S2 mediates the membrane fusion. In this process, heptad repeats 1 and 2 (HR1 and HR2) of the S protein assemble into a complex called six-helix bundle (6-HB) fusion core structure, which represents a key membrane fusion architecture. Importantly, the S protein is considered a key component of vaccines against coronavirus infection, including severe acute respiratory syndrome (SARS).
Gene ID	14254594
UniProt ID	W6A028
Alternative Names	MERS Spike gylcoprotein, MERS S glycoprotein, MERS E2, MERS Peplomer protein, MERS Spike protein S2, MERS-RBD, Middle East respiratory syndrome coronavirus spike
Related Disease	Middle East Respiratory Syndrome

Antigen Details

Full Name

Spike glycoprotein

Introduction

The MERS-CoV spike (S) protein is a type I transmembrane glycoprotein which contains 1353 amino acids and can be cleaved into two subunit S1 and S2. It forms large protruding spikes on the surface of the virus. The S1 subunit which contains RBD is responsible for binding to Dipeptidyl peptidase 4, which serves as the host cell receptor of MERS-CoV and S2 mediates the membrane fusion. In this process, heptad repeats 1 and 2 (HR1 and HR2) of the S protein assemble into a complex called six-helix bundle (6-HB) fusion core structure, which represents a key membrane fusion architecture. Importantly, the S protein is considered a key component of vaccines against coronavirus infection, including severe acute respiratory syndrome (SARS).

Gene ID

14254594

UniProt ID

W6A028

Alternative Names

MERS Spike gylcoprotein, MERS S glycoprotein, MERS E2, MERS Peplomer protein, MERS Spike protein S2, MERS-RBD, Middle East respiratory syndrome coronavirus spike

Related Disease

Middle East Respiratory Syndrome

Virus Details
Virus Family	Coronaviridae
Virus Species	MERS-COV
Virus Classification	Positive-sense RNA Virus

Virus Details

Virus Family

Coronaviridae

Virus Species

MERS-COV

Virus Classification

Positive-sense RNA Virus

Packaging, Storage & Formulations
Purification	Protein A affinity purified
Storage	Store at 4°C for short term. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.
Buffer	PBS
Form	Liquid
Preservative	0.02% Proclin 300.
Formulation	PBS with 0.02% Proclin 300.
Concentration	1 mg/mL
Purity	>95% as determined by SDS-PAGE

Packaging, Storage & Formulations

Purification

Protein A affinity purified

Storage

Store at 4°C for short term. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Buffer

PBS

Form

Liquid

Preservative

0.02% Proclin 300.

Formulation

PBS with 0.02% Proclin 300.

Concentration

1 mg/mL

Purity

>95% as determined by SDS-PAGE

Application Notes
Application	; Block; Neut;
Application Notes	This antibody was created using our proprietary engineered Fc domain containing key point mutations that abrogate binding to Fc gamma receptors. This antibody blocks the binding of MERS-CoV spike protein to DPP4 receptor by binding to the aa 349-590 in the S1 domain of the MERS-CoV.The optimal working dilutions should be determined by the end user.

Application Notes

Application

; Block; Neut;

Application Notes

This antibody was created using our proprietary engineered Fc domain containing key point mutations that abrogate binding to Fc gamma receptors.
This antibody blocks the binding of MERS-CoV spike protein to DPP4 receptor by binding to the aa 349-590 in the S1 domain of the MERS-CoV.The optimal working dilutions should be determined by the end user.

Application

3D IHC3D Immunohistochemistry

ActivActivation Assay

AgonistAgonist

ALPHAAmplified Luminescent Proximity Homogeneous Assay

AntagonistAntagonist

ADCCAntibody-dependent Cellular Cytotoxicity Assay

ApopApoptosis Assay

BABioAssay

BIBioimaging

BlockBlocking

CACell-based Assay/Cellular Assay

ChIPChromatin Immunoprecipitation

CMCDComplement Mediated Cell Depletion

CDCComplement-dependent Cytotoxicity Assay

CostimCostimulation Assay

CytCytotoxicity Assay

DepDepletion Assay

DBDot Blot/Slot blot

ECLElectrochemiluminescence

EMElectron Microscopy

EMSAElectrophoretic Mobility Shift Assay

ELISAEnzyme-Linked Immunosorbent Assay

ELISpotEnzyme-linked Immunosorbent Spot

FCFlow Cytometry

FIAFluorescence Immunoassay

FRETFluorescence Resonance Energy Transfer Microscopy

FuncSFunctional Assay

GSGel Super Shift Assay

HAHemagglutination Assay

IAImmunoassay

IBImmunoblotting

ICCImmunocytochemistry

IDImmunodiffusion

IEPImmunoelectrophoresis

IFImmunofluorescence

IHCImmunohistochemistry

IHC-FrImmunohistochemistry-Frozen

IHC-PImmunohistochemistry-Paraffin

REImmunohistology-Resin Sections

IMImmunomicroscopy

IPImmunoprecipitation

IRMAImmunoradiometric Assay

ISHIn Situ Hybridization

InhibInhibition Assay

ICFCIntracellular Staining for Flow Cytometry

Live-cell imagingLive-cell Imaging Assay

CyTOFMass Cytometry

MeDIPMethylated DNA Immunoprecipitation

MAMicroagglutination Assay

NeutNeutralization Assay

PAPeptide Array

PPProtein Purification

PGProteogenomics

RIRadial Immunodiffusion

RIARadioimmunoassay

StimStimulation Assay

SPRSurface Plasmon Resonance

TCAT-Cell Activation Assay

TRFTime-Resolved Fluorescence

TCTissue Culture

TBTurbidimetry

WBWestern Blot

HIHemagglutinin Inhibition

FLISAFluorescence-linked immunosorbent assay

CtrlControl

Dual ISH-IHCDual In Situ Hybridization-Immunohistochemistry

ICFCIntracellular Staining for Flow Cytometry

KDKnockdown Validated

BLIBio-Layer Interferometry

LFIALateral-Flow Immunochromatographic Assay

CryCrystallization

LFALateral flow assay