Lenti-VSV GP Pseudovirus [Luciferase, MLV Packaging System] (CAT#: PSV-099CQ)

Lenti-VSV GP Pseudovirus is produced by transfecting the cell line GP2-293 expressing express the MMLV Gag and Pol gene products with the pLNHX-Luciferase plasmids and a plasmid encoding the VSVG gene. Pseudovirus-containing supernatant was harvested 48 h following transfection. The supernatant was cleared of cell debris by centrifugation and filtered through a 0.45-μm-pore-size filter. The produced pseudoviruses carry VSV G proteins, which is applied for transgenic study and RNAi gene expression interference research.

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Product Details
Virus Packaging ProteinGP
Packaging SystemLentivirus
Packaging CellGP2-293
Reporter GeneLuciferase
Application NotesThe pseudovirus can be usd for the following applications:
1. Antibody neutralization assay, Serum neutralization assay.
2. As virus standard alternative in diagnostic assay or others.
3. Drug screening including vaccine development, viral entry inhibitor development.
4. As a safe virology tool to discover the virus entry mechanisms.
Titer>1^8 virus copies/mL
StorageStore at -80°C and stable for 6 months from the date of shipment, avoiding freeze/thaw cycles.
NoteThe pseudovirus can be used under BSL-2 conditions. Please reach out to our scientists for detailed instructions or guidelines of the pseudovirus.
Pseudovirus Details
Virus FamilyRhabdoviridae
Virus SpeciesVSV
Virus ClassificationNegative-sense RNA Virus
Related DiseaseVesicular stomatitis
IntroductionVesicular stomatitis virus (VSV) is a bullet-shaped, single-stranded negative sense RNA virus and belongs to the family Rhabdoviridae, genus Vesiculovirus. VSV structure is a virus. Structurally, it is composed of a lipid-glycoprotein envelope surrounding the nucleoprotein core. The envelope is composed of glycoprotein (G) protein and matrix (M) protein. G protein mediates viral attachment to an LDL receptor (LDLR) or an LDLR family member present on the host cell. M protein not only is a structural protein but likely responsible for the pathogenesis by inhibiting innate interferon responses.
Alternative NamesVSV-G; VSIVgp4 glycoprotein; G glycoprotein;VSV
Gene ID1489834
All products and services are intended for Research Use Only, and NOT to be used in diagnostic or therapeutic procedures.

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