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MBGV GP Pseudovirus is produced by cotransfecting 293T cells with the mammalian expression vector pCMV4neo encoding MBGV GP protein and HIV-1 NL4-3 carrying a luciferase reporter gene. Pseudovirus-containing supernatant was harvested 24 h following transfection. The supernatant was cleared of cell debris by centrifugation and filtered through a 0.45-μm-pore-size filter. The produced pseudoviruses carry MBGV GP proteins, which is a powerful tool for MBGV vaccine and entry inhibitor research.
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|Virus Packaging Protein||GP|
|Application Notes||The pseudovirus can be usd for the following applications:|
1. Antibody neutralization assay, Serum neutralization assay.
2. As virus standard alternative in diagnostic assay or others.
3. Drug screening including vaccine development, viral entry inhibitor development.
4. As a safe virology tool to discover the virus entry mechanisms.
|Titer||>1^8 virus copies/mL|
|Storage||Store at -80°C and stable for 6 months from the date of shipment, avoiding freeze/thaw cycles.|
|Note||The pseudovirus can be used under BSL-2 conditions. Please reach out to our scientists for detailed instructions or guidelines of the pseudovirus.|
|Virus Classification||Negative-sense RNA Virus|
|Introduction||Marburg viruses (MBGV) are enveloped, single negative-stranded RNA viruses belonging to the family Filoviridae. The viruses are the most deadly human-disease-causing agents (biosafety level-4 pathogens ). MBGV infection in humans is often associated with severe hemorrhagic fever and high mortality. MBGV contains a transmembrane glycoprotein (GP) which is heavily glycosylated with both N-linked carbohydrates (including high-mannose oligosaccharides) and O-linked carbohydrates. GP forms the trimeric spikes that occupy the entire surface of the virion. It is responsible for cellular binding and fusion. Therefore, GP is a key target for preventing MBGV infections. Ssubstances that directly interact with GP might interfere with filovirus infection by preventing viral entry into and/or fusion with the target cells.|
|Alternative Names||GP; MARV GP; MARV_gp4|