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Cat#: VRS-0124-YT5287
Lenti-LACV G1/G2 Pseudovirus [β-Galactosidase, MLV Packaging System]
Lenti-LACV G1/G2 Pseudovirus is produced by cotransfecting 293T cells with the plasmid inserting G1 and G2 proteins, pcGP (MLV gag-pol), and a β-Galactosidase gene. Pseudovirus-containing supernatant was harvested 48 h following transfection. The supernatant was cleared of cell debris by centrifugation and filtered through a 0.45-μm-pore-size filter. The produced pseudoviruses carrying LACV G1 and G2 proteins is a powerful tool for studying mechanism of virus entry, antiviral mechanism and screening antivirus inhibitor.
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- Product Summary
- Packaging, Storage & Formulations
- Target Details
Product Summary
Type : | Pseudovirus |
Virus Name : | La Crosse Virus |
Virus Packaging Protein : | G1/G2 |
Packaging System : | MLV Packaging System |
Packaging Cell : | 293T |
Reporter Gene : | β-Galactosidase |
Titer : | >1x 108 virus copies/mL |
Application Notes : | The pseudovirus can be usd for the following applications: 1. Antibody neutralization assay, Serum neutralization assay. 2. As virus standard alternative in diagnostic assay or others. 3. Drug screening including vaccine development, viral entry inhibitor development. 4. As a safe virology tool to discover the virus entry mechanisms. |
Packaging, Storage & Formulations
Form : | Liquid |
Storage : | Store at -80°C and stable for 6 months from the date of shipment, avoiding freeze/thaw cycles. |
Shipping : | Dry ice |
Note : | The pseudovirus can be used under BSL-2 conditions. Please reach out to our scientists for detailed instructions or guidelines of the pseudovirus. |
Target Details
Introduction : | La Crosse virus (LACV) is the causative agent of La Crosse encephalitis, a vector-borne disease of children in the United States. LACV is a member of the family Bunyaviridae, in the genus Bunyavirus. The Bunyaviridae are negative stranded RNA viruses with a tri-partite segmented genome composed of large (L), medium (M) and small (S) segments. The L segment encodes a polymerase protein (also called L), and the M and S segments encode, respectively, an open reading frame for the viral glycoproteins (G1 and G2), and in some genera, a non-structural protein, and the nucleocapsid protein (N) with another non-structural protein. |
Alternative Names : | LACV G1/G2 Pseudovirus; G1/G2 membrane polyprotein; M; La Crosse virus |
Gene ID : | 956555 |
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.