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Cat#: VRS-0124-YT5211
Lenti-hRSV F Pseudovirus [Luciferase, HIV-1 Packaging System]
Lenti-hRSV SH/G Pseudovirus is produced by cotransfecting 293T cells with the hRSV envelope protein expression construct pcDNA-hRSV-F, the pCMV-ΔR8.74 lentiviral Gag-Pol expression construct, and a luciferase transducing vector (pWPI_F-Luc_BLR). Pseudovirus-containing supernatant was harvested 30 h following transfection. The supernatant was cleared of cell debris by centrifugation and filtered through a 0.45-μm-pore-size filter. The produced pseudoviruses carrying hRSV SH/G is a powerful tool for studying mechanism of virus entry, antiviral mechanism, neutralization antibody assay, and screening antivirus inhibitor.
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- Product Summary
- Packaging, Storage & Formulations
- Target Details
Product Summary
| Type : | Pseudovirus |
| Virus Name : | Human Orthopneumovirus; Human Respiratory Syncytial Virus |
| Virus Packaging Protein : | F |
| Packaging System : | HIV-1 Packaging System |
| Packaging Cell : | 293T |
| Reporter Gene : | Luciferase |
| Titer : | >1x 108 virus copies/mL |
| Application Notes : | The pseudovirus can be usd for the following applications: 1. Antibody neutralization assay, Serum neutralization assay. 2. As virus standard alternative in diagnostic assay or others. 3. Drug screening including vaccine development, viral entry inhibitor development. 4. As a safe virology tool to discover the virus entry mechanisms. |
Packaging, Storage & Formulations
| Form : | Liquid |
| Storage : | Store at -80°C and stable for 6 months from the date of shipment, avoiding freeze/thaw cycles. |
| Shipping : | Dry ice |
| Note : | The pseudovirus can be used under BSL-2 conditions. Please reach out to our scientists for detailed instructions or guidelines of the pseudovirus. |
Target Details
| Introduction : | Human respiratory syncytial virus (hRSV) is an enveloped, nonsegmented, negative-strand RNA virus classified in the family Paramyxoviridae. It causes upper and lower respiratory tract disease in children and elderly and immunosuppressed persons. hRSV possesses three envelope proteins, the fusion glycoprotein (F), the attachment glycoprotein (G), and the small hydrophobic protein (SH). The F protein directs membrane fusion independently of other viral envelope proteins. The hRSV-G protein is highly variable and heavily glycosylated. It is important for virus attachment, primarily through interaction with glycosaminoglycans. The hRSV-SH protein forms oligomeric channel-like structures with 5- or 6-fold symmetry with a nonselective cation channel activity in mammalian cells. |
| Alternative Names : | hRSV F Pseudovirus; F; hRSV; Fusion protein; Rsvgp08 |
| Gene ID : | 1494475 |
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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