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This human Monoclonal antibody (XF0050) is a human IgG1 antiobdy specific for SARS-CoV-2 S RBD domain. It can inhibit or neutralize the interaction between SARS-CoV-2 spike protein RBD domain and ACE2. The antibody can be used for applications: WB, ELISA, Neut. The antibody is for research use only and is not approved for use in humans or in clinical diagnosis.
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|Product Category||Primary antibody|
|Application||WB, ELISA, Neut|
|Specificity||This human IgG1 antibody recognizes the SARS-CoV-2 spike protein RBD domain and can inhibit or neutralize the interaction between S RBD and ACE2. The reactivity with other coronavirus hasn't been tested.|
|Full Name||Spike glycoprotein|
|Introduction||The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.|
|Alternative Names||S, S glycoprotein, Spike, Spike glycoprotein, Surface glycoprotein, SARS-CoV-2, SARS-CoV-2 S, SARS-CoV-2 Spike, SARS-CoV-2 Surface glycoprotein, 2019-nCoV, 2019-nCoV S, 2019-nCoV Spike, 2019-nCoV Surface glycoprotein, COVID-19, COVID-19 S, COVID-19 Spike, COVID-19 Surface glycoprotein|
|Virus Classification||Positive-sense RNA Virus|
|Packaging, Storage & Formulations|
|Storage||Store at 4°C for short term (12 months in lyophilized state). Store at -20°C for long term (-80°C for 3 years under sterile conditions after reconstitution. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.|
|Buffer||PBS (pH 7.4)|
|Formulation||Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally trehalose is added as protectant before lyophilization.|
|Purity||> 95% as determined by SDS|
|Application||WB, ELISA, Neut|
|Application Notes||The optimal working dilutions should be determined by the end user.|