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This Human Monoclonal antibody is specific for MERS-CoV Spike protein. The antibody can be used for applications: Block,Neut. The antibody is for Middle East Respiratory Syndromedisease research use only and is not approved for use in humans or in clinical diagnosis.
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|Product Category||Primary Antibody|
|Species Reactivity||; MERS-CoV;|
|Application||; Block; Neut;|
|Specificity||This antibody specifcally binds the amino acids 349-590 in the S1 domain of the MERS-CoV.|
|Immunogen||The original antibody was generated by isolating neutralizing antibodies from a non-immune human Ab-phage library using a novel panning strategy.|
|Isotype||IgG1(Fc Engineered), Kappa|
|Full Name||Spike glycoprotein|
|Introduction||The MERS-CoV spike (S) protein is a type I transmembrane glycoprotein which contains 1353 amino acids and can be cleaved into two subunit S1 and S2. It forms large protruding spikes on the surface of the virus. The S1 subunit which contains RBD is responsible for binding to Dipeptidyl peptidase 4, which serves as the host cell receptor of MERS-CoV and S2 mediates the membrane fusion. In this process, heptad repeats 1 and 2 (HR1 and HR2) of the S protein assemble into a complex called six-helix bundle (6-HB) fusion core structure, which represents a key membrane fusion architecture. Importantly, the S protein is considered a key component of vaccines against coronavirus infection, including severe acute respiratory syndrome (SARS).|
|Alternative Names||MERS Spike gylcoprotein, MERS S glycoprotein, MERS E2, MERS Peplomer protein, MERS Spike protein S2, MERS-RBD, Middle East respiratory syndrome coronavirus spike|
|Related Disease||Middle East Respiratory Syndrome|
|Virus Classification||Positive-sense RNA Virus|
|Packaging, Storage & Formulations|
|Purification||Protein A affinity purified|
|Storage||Store at 4°C for short term. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.|
|Preservative||0.02% Proclin 300.|
|Formulation||PBS with 0.02% Proclin 300.|
|Purity||>95% as determined by SDS-PAGE|
|Application||; Block; Neut;|
|Application Notes||This antibody was created using our proprietary engineered Fc domain containing key point mutations that abrogate binding to Fc gamma receptors. This antibody blocks the binding of MERS-CoV spike protein to DPP4 receptor by binding to the aa 349-590 in the S1 domain of the MERS-CoV. It is a neutralizing antibody with IC50 value ranging from 1.25 to 2 μg/mL. The optimal working dilutions should be determined by the end user.|