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Creative Biolabs has been setting the standard in quality virology research reagents for over 10 years.
Human HSPG-overexpressed 293T pseudohost is produced by transfection 293T cells with a lentiviral vector encoding human HSPG proteins. This stable cell line express human HSPG proteins on the cell surface, and it can be used for drug/vaccine/viral inhibitor screening, neutralizing antibody screening and serum neutralization assay.
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|Application Notes||The pseudohost can be usd for the following applications:|
1. Antibody neutralization assay, Serum neutralization assay.
3. Drug screening including vaccine development, viral entry inhibitor development.
4. Virus entry mechanism discovery.
|Mycoplasma Testing||The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.|
|Quality Control||Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.|
|Storage||Cells must be thawed immediately upon receipt and grown according to handling procedures to ensure cell viability and proper assay performance. DO NOT FREEZE the cells upon receipt as it may result in irreversible damage to the cell line. After recovery, the cells can be stored at -80°C or liquid nitrogen (-150°C to -190°C) for long term.|
|Note||We cannot guarantee cell viability if the cells are not thawed immediately upon receipt. Avoid multiple freeze/thaw cycles.|
|Introduction||Heparan sulfate proteoglycans (HSPGs) are complex molecules composed of a core protein with covalently attached glycosaminoglycans chains, especially heparan sulfate. HSPGs are involved in a wide variety of biological phenomena, including organogenesis, angiogenesis, growth factors/cytokine actions, wound healing, and cell adhesion. Moreover, they are implicated as primary host cell receptors for many viruses, although most of them depend on secondary receptor proteins for efficient internalization. HSPGs were reported to play a critical role in the binding of HPVs to the cell surface.|
|Alternative Names||HSPG; CD362; HSPG1; SYND2|