Human ACE2 Overexpressed 293T Cell Line Pseudohost (CAT#: PSH-001F)

Human ACE2-overexpressed 293T pseudohost is produced by transfection 293T cells with a lentiviral vector encoding human angiotensin-converting enzyme 2 (hACE2). This stable cell line express hACE2 on the cell surface, and it can be used for drug/vaccine/viral inhibitor screening, neutralizing antibody screening and serum neutralization assay.

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Product Details
Cell Type293T
Application NotesThe pseudohost can be usd for the following applications:
1. Antibody neutralization assay, Serum neutralization assay.
3. Drug screening including vaccine development, viral entry inhibitor development.
4. Virus entry mechanism discovery.
Related DiseaseCOVID-19; SARS
Mycoplasma TestingThe cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Neutralization AssayValidated
Quality ControlCultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.
StorageCells must be thawed immediately upon receipt and grown according to handling procedures to ensure cell viability and proper assay performance. DO NOT FREEZE the cells upon receipt as it may result in irreversible damage to the cell line. After recovery, the cells can be stored at -80°C or liquid nitrogen (-150°C to -190°C) for long term.
NoteWe cannot guarantee cell viability if the cells are not thawed immediately upon receipt. Avoid multiple freeze/thaw cycles.
Receptor Details
Infection ReceptorACE2
IntroductionThe protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2 (COVID-19 virus).
Gene ID59272
Alternative NamesACE2, ACEH, Angiotensin I converting enzyme 2
All products and services are intended for Research Use Only, and NOT to be used in diagnostic or therapeutic procedures.

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