Human ACE2 Overexpressed 293T Cell Line Pseudohost

Datasheet

MSDS

Certificate of Analysis Lookup

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Lot Number

Product Details
Cell Type	293T
Application Notes	The pseudohost can be usd for the following applications: 1. Antibody neutralization assay, Serum neutralization assay. 3. Drug screening including vaccine development, viral entry inhibitor development. 4. Virus entry mechanism discovery.
Related Disease	COVID-19; SARS
Mycoplasma Testing	The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Neutralization Assay	Validated
Quality Control	Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.
Storage	Cells must be thawed immediately upon receipt and grown according to handling procedures to ensure cell viability and proper assay performance. DO NOT FREEZE the cells upon receipt as it may result in irreversible damage to the cell line. After recovery, the cells can be stored at -80°C or liquid nitrogen (-150°C to -190°C) for long term.
Note	We cannot guarantee cell viability if the cells are not thawed immediately upon receipt. Avoid multiple freeze/thaw cycles.

Product Details

Cell Type

293T

Application Notes

The pseudohost can be usd for the following applications:
1. Antibody neutralization assay, Serum neutralization assay.
3. Drug screening including vaccine development, viral entry inhibitor development.
4. Virus entry mechanism discovery.

Related Disease

COVID-19; SARS

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Neutralization Assay

Validated

Quality Control

Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.

Storage

Cells must be thawed immediately upon receipt and grown according to handling procedures to ensure cell viability and proper assay performance. DO NOT FREEZE the cells upon receipt as it may result in irreversible damage to the cell line. After recovery, the cells can be stored at -80°C or liquid nitrogen (-150°C to -190°C) for long term.

Note

We cannot guarantee cell viability if the cells are not thawed immediately upon receipt. Avoid multiple freeze/thaw cycles.

Receptor Details
Infection Receptor	ACE2
Introduction	The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2 (COVID-19 virus).
Gene ID	59272
Alternative Names	ACE2, ACEH, Angiotensin I converting enzyme 2

Receptor Details

Infection Receptor

ACE2

Introduction

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2 (COVID-19 virus).

Gene ID

59272

Alternative Names

ACE2, ACEH, Angiotensin I converting enzyme 2

Application

3D IHC3D Immunohistochemistry

ActivActivation Assay

AgonistAgonist

ALPHAAmplified Luminescent Proximity Homogeneous Assay

AntagonistAntagonist

ADCCAntibody-dependent Cellular Cytotoxicity Assay

ApopApoptosis Assay

BABioAssay

BIBioimaging

BlockBlocking

CACell-based Assay/Cellular Assay

ChIPChromatin Immunoprecipitation

CMCDComplement Mediated Cell Depletion

CDCComplement-dependent Cytotoxicity Assay

CostimCostimulation Assay

CytCytotoxicity Assay

DepDepletion Assay

DBDot Blot/Slot blot

ECLElectrochemiluminescence

EMElectron Microscopy

EMSAElectrophoretic Mobility Shift Assay

ELISAEnzyme-Linked Immunosorbent Assay

ELISpotEnzyme-linked Immunosorbent Spot

FCFlow Cytometry

FIAFluorescence Immunoassay

FRETFluorescence Resonance Energy Transfer Microscopy

FuncSFunctional Assay

GSGel Super Shift Assay

HAHemagglutination Assay

IAImmunoassay

IBImmunoblotting

ICCImmunocytochemistry

IDImmunodiffusion

IEPImmunoelectrophoresis

IFImmunofluorescence

IHCImmunohistochemistry

IHC-FrImmunohistochemistry-Frozen

IHC-PImmunohistochemistry-Paraffin

REImmunohistology-Resin Sections

IMImmunomicroscopy

IPImmunoprecipitation

IRMAImmunoradiometric Assay

ISHIn Situ Hybridization

InhibInhibition Assay

ICFCIntracellular Staining for Flow Cytometry

Live-cell imagingLive-cell Imaging Assay

CyTOFMass Cytometry

MeDIPMethylated DNA Immunoprecipitation

MAMicroagglutination Assay

NeutNeutralization Assay

PAPeptide Array

PPProtein Purification

PGProteogenomics

RIRadial Immunodiffusion

RIARadioimmunoassay

StimStimulation Assay

SPRSurface Plasmon Resonance

TCAT-Cell Activation Assay

TRFTime-Resolved Fluorescence

TCTissue Culture

TBTurbidimetry

WBWestern Blot

HIHemagglutinin Inhibition

FLISAFluorescence-linked immunosorbent assay

CtrlControl

Dual ISH-IHCDual In Situ Hybridization-Immunohistochemistry

ICFCIntracellular Staining for Flow Cytometry

KDKnockdown Validated

BLIBio-Layer Interferometry

LFIALateral-Flow Immunochromatographic Assay

CryCrystallization

LFALateral flow assay