Chimeric (Mouse/Human) Anti-SARS-CoV-2 S Monoclonal Antibody (XF0035)

Datasheet

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Lot Number

Product Summary
Product Category	Primary Antibody
Target	S
Host	Chimeric (Mouse/Human)
Species Reactivity	SARS-CoV-2
Clonality	Monoclonal
Application	ELISA, IHC-P, FC, ICC, IF, IP

Product Summary

Product Category

Primary Antibody

Target

Host

Chimeric (Mouse/Human)

Species Reactivity

SARS-CoV-2

Clonality

Monoclonal

Application

ELISA, IHC-P, FC, ICC, IF, IP

Product Details
Specificity	This antibody reacts with spike S1 protein of SARS-CoV-2 (also known as 2019-nCoV), it also has cross-reactivity in ELISA with SARS-CoV Spike S1.
Clone	XF0035
Immunogen	Recombinant SARS-CoV Spike RBD Protein
Isotype	IgG1
Conjugation	Unconjugated

Product Details

Specificity

This antibody reacts with spike S1 protein of SARS-CoV-2 (also known as 2019-nCoV), it also has cross-reactivity in ELISA with SARS-CoV Spike S1.

Clone

XF0035

Immunogen

Recombinant SARS-CoV Spike RBD Protein

Isotype

IgG1

Conjugation

Unconjugated

Antigen Details
Full Name	Spike glycoprotein
Introduction	The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
Gene ID	43740568
Alternative Names	S, S glycoprotein, Spike, Spike glycoprotein, Surface glycoprotein, SARS-CoV-2, SARS-CoV-2 S, SARS-CoV-2 Spike, SARS-CoV-2 Surface glycoprotein, 2019-nCoV, 2019-nCoV S, 2019-nCoV Spike, 2019-nCoV Surface glycoprotein, COVID-19, COVID-19 S, COVID-19 Spike, COVID-19 Surface glycoprotein
Related Disease	COVID-19

Antigen Details

Full Name

Spike glycoprotein

Introduction

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

Gene ID

43740568

Alternative Names

S, S glycoprotein, Spike, Spike glycoprotein, Surface glycoprotein, SARS-CoV-2, SARS-CoV-2 S, SARS-CoV-2 Spike, SARS-CoV-2 Surface glycoprotein, 2019-nCoV, 2019-nCoV S, 2019-nCoV Spike, 2019-nCoV Surface glycoprotein, COVID-19, COVID-19 S, COVID-19 Spike, COVID-19 Surface glycoprotein

Related Disease

COVID-19

Virus Details
Virus Family	Coronaviridae
Virus Species	SARS-CoV-2
Virus Classification	Positive-sense RNA Virus

Virus Details

Virus Family

Coronaviridae

Virus Species

SARS-CoV-2

Virus Classification

Positive-sense RNA Virus

Packaging, Storage & Formulations
Purification	Protein A
Storage	Store at 4°C for short term. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.
Buffer	PBS
Form	Liquid
Purity	˃ 95% as determined by SDS-PAGE

Packaging, Storage & Formulations

Purification

Protein A

Storage

Store at 4°C for short term. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Buffer

PBS

Form

Liquid

Purity

˃ 95% as determined by SDS-PAGE

Application Notes
Application	ELISA, IHC-P, FC, ICC, IF, IP
Application Notes	ELISA: 1:5000-1:10000 The optimal working dilutions should be determined by the end user.

Application Notes

Application

ELISA, IHC-P, FC, ICC, IF, IP

Application Notes

ELISA: 1:5000-1:10000
The optimal working dilutions should be determined by the end user.

Application

3D IHC3D Immunohistochemistry

ActivActivation Assay

AgonistAgonist

ALPHAAmplified Luminescent Proximity Homogeneous Assay

AntagonistAntagonist

ADCCAntibody-dependent Cellular Cytotoxicity Assay

ApopApoptosis Assay

BABioAssay

BIBioimaging

BlockBlocking

CACell-based Assay/Cellular Assay

ChIPChromatin Immunoprecipitation

CMCDComplement Mediated Cell Depletion

CDCComplement-dependent Cytotoxicity Assay

CostimCostimulation Assay

CytCytotoxicity Assay

DepDepletion Assay

DBDot Blot/Slot blot

ECLElectrochemiluminescence

EMElectron Microscopy

EMSAElectrophoretic Mobility Shift Assay

ELISAEnzyme-Linked Immunosorbent Assay

ELISpotEnzyme-linked Immunosorbent Spot

FCFlow Cytometry

FIAFluorescence Immunoassay

FRETFluorescence Resonance Energy Transfer Microscopy

FuncSFunctional Assay

GSGel Super Shift Assay

HAHemagglutination Assay

IAImmunoassay

IBImmunoblotting

ICCImmunocytochemistry

IDImmunodiffusion

IEPImmunoelectrophoresis

IFImmunofluorescence

IHCImmunohistochemistry

IHC-FrImmunohistochemistry-Frozen

IHC-PImmunohistochemistry-Paraffin

REImmunohistology-Resin Sections

IMImmunomicroscopy

IPImmunoprecipitation

IRMAImmunoradiometric Assay

ISHIn Situ Hybridization

InhibInhibition Assay

ICFCIntracellular Staining for Flow Cytometry

Live-cell imagingLive-cell Imaging Assay

CyTOFMass Cytometry

MeDIPMethylated DNA Immunoprecipitation

MAMicroagglutination Assay

NeutNeutralization Assay

PAPeptide Array

PPProtein Purification

PGProteogenomics

RIRadial Immunodiffusion

RIARadioimmunoassay

StimStimulation Assay

SPRSurface Plasmon Resonance

TCAT-Cell Activation Assay

TRFTime-Resolved Fluorescence

TCTissue Culture

TBTurbidimetry

WBWestern Blot

HIHemagglutinin Inhibition

FLISAFluorescence-linked immunosorbent assay

CtrlControl

Dual ISH-IHCDual In Situ Hybridization-Immunohistochemistry

ICFCIntracellular Staining for Flow Cytometry

KDKnockdown Validated

BLIBio-Layer Interferometry

LFIALateral-Flow Immunochromatographic Assay

CryCrystallization

LFALateral flow assay