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This Chimeric (Human/Rabbit) Monoclonal antibody (XF0006) is specific for SARS-CoV S protein. The antibody can be used for applications: ELISA, Neut. The antibody is for Severe acute respiratory syndrome (SARS) disease research use only and is not approved for use in humans or in clinical diagnosis.
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|Product Category||Primary Antibody|
|Specificity||This antibody reacts with spike protein of SARS-CoV, inhibiting the binding of the glycoprotein to human ACE2.|
|Immunogen||The antibody was developed by immortalizing IgG-expressing B cells from recovered SARS patients|
|Full Name||Spike glycoprotein|
|Introduction||The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.|
|Alternative Names||S, S glycoprotein, Spike, Spike glycoprotein, E2 glycoprotein precursor, Surface glycoprotein, SARS-CoV, SARS-CoV S, SARS-CoV Spike, Coronavirus S, Coronavirus Spike|
|Related Disease||Severe acute respiratory syndrome (SARS)|
|Virus Classification||Positive-sense RNA Virus|
|Packaging, Storage & Formulations|
|Purification||Protein A affinity purified|
|Storage||Store at 4°C for short term (stable for 3 months). Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.|
|Preservative||0.02% Proclin 300|
|Formulation||PBS with 0.02% Proclin 300|
|Purity||˃ 95% as determined by SDS-PAGE|
|Application Notes||The optimal working dilutions should be determined by the end user.|
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