Human CD81 Overexpressed CHO Cells Line Pseudohost (CAT#: PSH-010CQ)

Human CD81-overexpressed CHO pseudohost is produced by transfection CHO cells with a lentiviral vector encoding human CD81 proteins. This stable cell line express human CD81 proteins on the cell surface, and it can be used for drug/vaccine/viral inhibitor screening, neutralizing antibody screening and serum neutralization assay.

Specific Inquiry

  • Size:

(Note: Please select the requirements to fit you project. If you have any other requirements, please specify. If no specific requirements, you don't need to select any button.)

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number

Product Details
Cell TypeCHO
Application NotesThe pseudohost can be usd for the following applications:
1. Antibody neutralization assay, Serum neutralization assay.
3. Drug screening including vaccine development, viral entry inhibitor development.
4. Virus entry mechanism discovery.
Related DiseaseHCV
Mycoplasma TestingThe cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Neutralization AssayValidated
Quality ControlCultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.
StorageCells must be thawed immediately upon receipt and grown according to handling procedures to ensure cell viability and proper assay performance. DO NOT FREEZE the cells upon receipt as it may result in irreversible damage to the cell line. After recovery, the cells can be stored at -80°C or liquid nitrogen (-150°C to -190°C) for long term.
NoteWe cannot guarantee cell viability if the cells are not thawed immediately upon receipt. Avoid multiple freeze/thaw cycles.
Receptor Details
Infection ReceptorCD81
IntroductionCD81 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. This protein plays a critical role in Hepatitis C attachment and/or cell entry by interacting with virus' E1/E2 glycoproteins heterodimer. The large extracellular loop of CD81 binds the hepatitis E2 glycoprotein dimer. HCV-E2 and CD81 binding Kd is 1.8 nM. HCV-E2 engaged CD81 is only 30% internalized after 12hr, suggesting CD81 may be primarily an attachment receptor for HCV.
Gene ID975
Alternative NamesS5.7; CVID6; TAPA1; TSPAN28
All products and services are intended for Research Use Only, and NOT to be used in diagnostic or therapeutic procedures.

Related products

Application

3D IHC3D Immunohistochemistry
ActivActivation Assay
AgonistAgonist
ALPHAAmplified Luminescent Proximity Homogeneous Assay
AntagonistAntagonist
ADCCAntibody-dependent Cellular Cytotoxicity Assay
ApopApoptosis Assay
BABioAssay
BIBioimaging
BlockBlocking
CACell-based Assay/Cellular Assay
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CDCComplement-dependent Cytotoxicity Assay
CostimCostimulation Assay
CytCytotoxicity Assay
DepDepletion Assay
DBDot Blot/Slot blot
ECLElectrochemiluminescence
EMElectron Microscopy
EMSAElectrophoretic Mobility Shift Assay
ELISAEnzyme-Linked Immunosorbent Assay
ELISpotEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FIAFluorescence Immunoassay
FRETFluorescence Resonance Energy Transfer Microscopy
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination Assay
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IEPImmunoelectrophoresis
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology-Resin Sections
IMImmunomicroscopy
IPImmunoprecipitation
IRMAImmunoradiometric Assay
ISHIn Situ Hybridization
InhibInhibition Assay
ICFCIntracellular Staining for Flow Cytometry
Live-cell imagingLive-cell Imaging Assay
CyTOFMass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MAMicroagglutination Assay
NeutNeutralization Assay
PAPeptide Array
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation Assay
SPRSurface Plasmon Resonance
TCAT-Cell Activation Assay
TRFTime-Resolved Fluorescence
TCTissue Culture
TBTurbidimetry
WBWestern Blot
HIHemagglutinin Inhibition
FLISAFluorescence-linked immunosorbent assay
CtrlControl
Dual ISH-IHCDual In Situ Hybridization-Immunohistochemistry
ICFCIntracellular Staining for Flow Cytometry
KDKnockdown Validated
BLIBio-Layer Interferometry
LFIALateral-Flow Immunochromatographic Assay
CryCrystallization
LFALateral flow assay

Be the first to learn more about exclusive virology antibody products, services and latest updates.

USA

Europe

Copyright © 2021 Creative Biolabs. All Rights Reserved.
Inquiry Basket