Human CD4 Overexpressed CHO Cell Line Pseudohost (CAT#: PSH-002CQ)

Human CD4-overexpressed CHO pseudohost is produced by transfection CHO cells with a lentiviral vector encoding human CD4 proteins. This stable cell line express CD4 proteins on the cell surface, and it can be used for drug/vaccine/viral inhibitor screening, neutralizing antibody screening and serum neutralization assay.

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Product Details
Cell TypeCHO
Application NotesThe pseudohost can be usd for the following applications:
1. Antibody neutralization assay, Serum neutralization assay.
3. Drug screening including vaccine development, viral entry inhibitor development.
4. Virus entry mechanism discovery.
Related DiseaseHIV; AIDS
Mycoplasma TestingThe cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Neutralization AssayValidated
Quality ControlCultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.
StorageCells must be thawed immediately upon receipt and grown according to handling procedures to ensure cell viability and proper assay performance. DO NOT FREEZE the cells upon receipt as it may result in irreversible damage to the cell line. After recovery, the cells can be stored at -80°C or liquid nitrogen (-150°C to -190°C) for long term.
NoteWe cannot guarantee cell viability if the cells are not thawed immediately upon receipt. Avoid multiple freeze/thaw cycles.
Receptor Details
Infection ReceptorCD4
IntroductionCD4 (cluster of differentiation 4) is a glycoprotein found on the surface of immune cells such as T helper cells, monocytes, macrophages, and dendritic cells. It is a member of the immunoglobulin superfamily. HIV-1 uses CD4 to gain entry into host T-cells and achieves this through its viral envelope protein known as gp120. The binding to CD4 creates a shift in the conformation of gp120 allowing HIV-1 to bind to a co-receptor expressed on the host cell. These co-receptors are chemokine receptors CCR5 or CXCR4. Following a structural change in another viral protein (gp41), HIV inserts a fusion peptide into the host cell that allows the outer membrane of the virus to fuse with the cell membrane.
Gene ID920
Alternative NamesCD4; CD4mut
All products and services are intended for Research Use Only, and NOT to be used in diagnostic or therapeutic procedures.

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