Human CAR Overexpressed 293T Cells Line Pseudohost

Datasheet

MSDS

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Lot Number

Product Details
Cell Type	293T
Application Notes	The pseudohost can be usd for the following applications: 1. Antibody neutralization assay, Serum neutralization assay. 3. Drug screening including vaccine development, viral entry inhibitor development. 4. Virus entry mechanism discovery.
Related Disease	Epidemic keratoconjunctivitis; Respiratory illnesses; Viral pneumonia
Mycoplasma Testing	The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Neutralization Assay	Validated
Quality Control	Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.
Storage	Cells must be thawed immediately upon receipt and grown according to handling procedures to ensure cell viability and proper assay performance. DO NOT FREEZE the cells upon receipt as it may result in irreversible damage to the cell line. After recovery, the cells can be stored at -80°C or liquid nitrogen (-150°C to -190°C) for long term.
Note	We cannot guarantee cell viability if the cells are not thawed immediately upon receipt. Avoid multiple freeze/thaw cycles.

Product Details

Cell Type

293T

Application Notes

The pseudohost can be usd for the following applications:
1. Antibody neutralization assay, Serum neutralization assay.
3. Drug screening including vaccine development, viral entry inhibitor development.
4. Virus entry mechanism discovery.

Related Disease

Epidemic keratoconjunctivitis; Respiratory illnesses; Viral pneumonia

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Neutralization Assay

Validated

Quality Control

Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.

Storage

Cells must be thawed immediately upon receipt and grown according to handling procedures to ensure cell viability and proper assay performance. DO NOT FREEZE the cells upon receipt as it may result in irreversible damage to the cell line. After recovery, the cells can be stored at -80°C or liquid nitrogen (-150°C to -190°C) for long term.

Note

We cannot guarantee cell viability if the cells are not thawed immediately upon receipt. Avoid multiple freeze/thaw cycles.

Receptor Details
Infection Receptor	CAR
Introduction	Coxsackievirus and adenovirus receptor (CAR) is a protein that in humans is encoded by the CXADR gene. The protein encoded by this gene is a type I membrane receptor for group B coxsackie viruses and subgroup C adenoviruses. CAR protein is expressed in several tissues, including heart, brain, and, more generally, epithelial and endothelial cells. In cardiac muscle, CAR is localized to intercalated disc structures, which electrically and mechanically couple adjacent cardiomyocytes. It functions as a homophilic and heterophilic cell adhesion molecule through its interactions with extracellular matrix glycoproteins such as: fibronectin, agrin, laminin-1 and tenascin-R. In addition, it is thought to regulate the cytoskeleton through interactions with actin and microtubules. Moreover, its cytoplasmic domain contains putative phosphorylation sites and a PDZ-interaction motif which suggests a scaffolding role.
Gene ID	1525
Alternative Names	CAR; HCAR; CAR4/6

Receptor Details

Infection Receptor

CAR

Introduction

Coxsackievirus and adenovirus receptor (CAR) is a protein that in humans is encoded by the CXADR gene. The protein encoded by this gene is a type I membrane receptor for group B coxsackie viruses and subgroup C adenoviruses. CAR protein is expressed in several tissues, including heart, brain, and, more generally, epithelial and endothelial cells. In cardiac muscle, CAR is localized to intercalated disc structures, which electrically and mechanically couple adjacent cardiomyocytes. It functions as a homophilic and heterophilic cell adhesion molecule through its interactions with extracellular matrix glycoproteins such as: fibronectin, agrin, laminin-1 and tenascin-R. In addition, it is thought to regulate the cytoskeleton through interactions with actin and microtubules. Moreover, its cytoplasmic domain contains putative phosphorylation sites and a PDZ-interaction motif which suggests a scaffolding role.

Gene ID

1525

Alternative Names

CAR; HCAR; CAR4/6

Human CAR Overexpressed 293T Cells Line Pseudohost

Human CAR Overexpressed 293T Cells Line Pseudohost (CAT#: PSH-011CQ)

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