Bac-SARS-CoV-2 S Pseudovirus (CAT#: PSV-006F)

SARS-CoV-2 spike pseudovirus is generated by co-transfection 293T cells with a modified baculovirus vector, a fluorescent protein vector and a plasmid encoding SARS-CoV-2 S protein. The supernatants were harvested at 64 hours post transfection. The supernatants are centrifuged to remove cell debris, concentrated and passed through filter to get pseudovirus. The pseudovirus express SARS-CoV-2 spike protein on the surface, and also a fluorescent reporter which can be used to evaluate the SARS-CoV-2 infection when co-culture with human ACE2-overexpressed pseudohost. Pseudoviruses are useful virological tools because of their safety and versatility.

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Product Details
Virus Packaging ProteinS
Packaging SystemBaculovirus
Packaging Cell293T
Schematic diagram
Application NotesThe pseudovirus can be usd for the following applications:
1. Antibody neutralization assay, Serum neutralization assay.
2. As virus standard alternative in diagnostic assay or others.
3. Drug screening including vaccine development, viral entry inhibitor development.
4. As a safe virology tool to discover the virus entry mechanisms.
StorageStore at -80°C and stable for 1 year from the date of shipment, avoiding freeze/thaw cycles.
NoteThe pseudovirus can be used under BSL-1 conditions. Please reach out to our scientists for detailed instructions or guidelines of the pseudovirus.
Pseudovirus Details
Virus FamilyCoronaviridae
Virus SpeciesSARS-CoV-2
Virus ClassificationPositive-sense RNA Virus
Related DiseaseCOVID-19
IntroductionAn outbreak of a novel coronavirus disease (COVID-19; previously known as 2019-nCoV) was reported since last December, 2019. Now COVID-19 is an emerging, rapidly evolving situation, and has become a pandemic.SARS-CoV-2 consists of four structural proteins, including S (spike), E (envelope), M (membrane), and N (nucleocapsid) proteins. As an important envelope protein, S protein is identified as the protein responsible for virus entry into human cells, and S protein is the major target for anti-SARS-CoV-2 therapeutics discovery.
Alternative NamesSARS-CoV-2, 2019nCoV, COVID-19, S, Spike, Pseudovirus, Spike Pseudovirus, S Pseudovirus, SARS-CoV-2 Spike Pseudovirus, SARS-CoV-2 S Pseudovirus, COVID-19 Spike Pseudovirus, COVID-19 S Pseudovirus
Gene ID43740568
All products and services are intended for Research Use Only, and NOT to be used in diagnostic or therapeutic procedures.

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