Bac-SARS-CoV-2 S Pseudovirus

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• Bac-SARS-CoV-2 S Pseudovirus (CAT#: PSV-006F)

SARS-CoV-2 spike pseudovirus is generated by co-transfection 293T cells with a modified baculovirus vector, a fluorescent protein vector and a plasmid encoding SARS-CoV-2 S protein. The supernatants were harvested at 64 hours post transfection. The supernatants are centrifuged to remove cell debris, concentrated and passed through filter to get pseudovirus. The pseudovirus express SARS-CoV-2 spike protein on the surface, and also a fluorescent reporter which can be used to evaluate the SARS-CoV-2 infection when co-culture with human ACE2-overexpressed pseudohost. Pseudoviruses are useful virological tools because of their safety and versatility.

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Product Details
Virus Packaging Protein	S
Packaging System	Baculovirus
Packaging Cell	293T
Schematic diagram
Application Notes	The pseudovirus can be usd for the following applications: 1. Antibody neutralization assay, Serum neutralization assay. 2. As virus standard alternative in diagnostic assay or others. 3. Drug screening including vaccine development, viral entry inhibitor development. 4. As a safe virology tool to discover the virus entry mechanisms.
Storage	Store at -80°C and stable for 1 year from the date of shipment, avoiding freeze/thaw cycles.
Note	The pseudovirus can be used under BSL-1 conditions. Please reach out to our scientists for detailed instructions or guidelines of the pseudovirus.

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