Anti-SARS-CoV S Recombinant Antibody (VF0035) (CAT#: VRV-0035F)

This recombinant human monoclonal antibody (VF0035) is specific for SARS-CoV S protein recognition. The antibody can be used in applications: ELISA, Neut. The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. For any requirments, please specify and submit a inquiry for assistance.

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Product Summary
Product CategoryPrimary antibody
Species ReactivitySARS-CoV
ApplicationELISA, Neut
Product Details
SpecificityThis antibody recognizes Spike protein of SARS-CoV.
ImmunogenPurified S protein (Tor2; Genebank accession number: AY2741 19)
EpitopeConfirmational epitope
IsotypeIgG2, kappa
Antigen Details
Full NameSpike glycoprotein
IntroductionThe spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
Gene ID1489668
UniProt IDA7J8L4
Alternative NamesS, S glycoprotein, Spike, Spike glycoprotein, E2 glycoprotein precursor, Surface glycoprotein, SARS-CoV, SARS-CoV S, SARS-CoV Spike, Coronavirus S, Coronavirus Spike
Related DiseaseSevere acute respiratory syndrome (SARS)
Virus Details
Virus FamilyCoronaviridae
Virus SpeciesSARS-CoV
Virus ClassificationPositive-sense RNA Virus
Packaging, Storage & Formulations
PurificationPurified with Protein A or G affinity chromatography
StorageStore at 4°C for short term. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.
BufferPBS (pH 7.4), please specify if any other requirements.
FormLiquid and Lyophilized powder are both available.
PreservativePreservative-free, please specify if any other requirements.
FormulationPBS (pH 7.4) without any preservative, please specify if any other requirements.
Concentration1mg/mL, please specify if any other requirements.
Application Notes
ApplicationELISA, Neut
Application NotesThe optimal working dilutions should be determined by the end user.
All products and services are intended for Research Use Only, and NOT to be used in diagnostic or therapeutic procedures.
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