Certificate of Analysis Lookup
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Lot Number
Anti-SARS-CoV S Recombinant Antibody Fab Fragment (VF0025) (CAT#: VRV-0025F-Fab)
This recombinant human monoclonal antibody Fab fragment (VF0025) is specific for SARS-CoV S protein recognition. The antibody can be used in applications: ELISA, Neut. The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. For any requirments, please specify and submit a inquiry for assistance.
Specific Inquiry
(Note: Please select the requirements to fit you project. If you have any other requirements, please specify. If no specific requirements, you don't need to select any button.)
| Product Summary | |
|---|---|
| Product Category | Primary antibody |
| Target | S |
| Host | Human |
| Species Reactivity | ; SARS-CoV; |
| Clonality | Monoclonal |
| Application | ; ELISA; Neut; |
| Product Details | |
|---|---|
| Specificity | This antibody recognizes Spike protein of SARS-CoV. |
| Clone | VF0025 |
| Immunogen | The original antibody was isolated using blood sample derived from the recovered patient infected with SARS-CoV. |
| Isotype | IgG |
| Subtype | Fab |
| Antigen Details | |
|---|---|
| Full Name | Spike glycoprotein |
| Introduction | The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. |
| Gene ID | 1489668 |
| UniProt ID | A7J8L4 |
| Alternative Names | S, S glycoprotein, Spike, Spike glycoprotein, E2 glycoprotein precursor, Surface glycoprotein, SARS-CoV, SARS-CoV S, SARS-CoV Spike, Coronavirus S, Coronavirus Spike |
| Related Disease | Severe acute respiratory syndrome (SARS) |
| Virus Details | |
|---|---|
| Virus Family | Coronaviridae |
| Virus Species | SARS-CoV |
| Virus Classification | Positive-sense RNA Virus |
| Packaging, Storage & Formulations | |
|---|---|
| Purification | Purified with his tag |
| Storage | Store at 4°C for short term. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Buffer | PBS (pH 7.4), please specify if any other requirements. |
| Form | Liquid and Lyophilized powder are both available. |
| Preservative | Preservative-free, please specify if any other requirements. |
| Formulation | PBS (pH 7.4) without any preservative, please specify if any other requirements. |
| Concentration | 1mg/mL, please specify if any other requirements. |
| Application Notes | |
|---|---|
| Application | ; ELISA; Neut; |
| Application Notes | The optimal working dilutions should be determined by the end user. |
All products and services are intended for Research Use Only, and NOT to be used in diagnostic or therapeutic procedures.
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Application
3D IHC3D Immunohistochemistry
ActivActivation Assay
AgonistAgonist
ALPHAAmplified Luminescent Proximity Homogeneous Assay
AntagonistAntagonist
ADCCAntibody-dependent Cellular Cytotoxicity Assay
ApopApoptosis Assay
BABioAssay
BIBioimaging
BlockBlocking
CACell-based Assay/Cellular Assay
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CDCComplement-dependent Cytotoxicity Assay
CostimCostimulation Assay
CytCytotoxicity Assay
DepDepletion Assay
DBDot Blot/Slot blot
ECLElectrochemiluminescence
EMElectron Microscopy
EMSAElectrophoretic Mobility Shift Assay
ELISAEnzyme-Linked Immunosorbent Assay
ELISpotEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FIAFluorescence Immunoassay
FRETFluorescence Resonance Energy Transfer Microscopy
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination Assay
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IEPImmunoelectrophoresis
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology-Resin Sections
IMImmunomicroscopy
IPImmunoprecipitation
IRMAImmunoradiometric Assay
ISHIn Situ Hybridization
InhibInhibition Assay
ICFCIntracellular Staining for Flow Cytometry
Live-cell imagingLive-cell Imaging Assay
CyTOFMass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MAMicroagglutination Assay
NeutNeutralization Assay
PAPeptide Array
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation Assay
SPRSurface Plasmon Resonance
TCAT-Cell Activation Assay
TRFTime-Resolved Fluorescence
TCTissue Culture
TBTurbidimetry
WBWestern Blot
HIHemagglutinin Inhibition
FLISAFluorescence-linked immunosorbent assay
CtrlControl
Dual ISH-IHCDual In Situ Hybridization-Immunohistochemistry
ICFCIntracellular Staining for Flow Cytometry
KDKnockdown Validated
BLIBio-Layer Interferometry
LFIALateral-Flow Immunochromatographic Assay
CryCrystallization
LFALateral flow assay
