Anti-MERS-COV S Recombinant Antibody Fab Fragment (VR0260) (CAT#: VRV-0534R)

This recombinant Human monoclonal antibody Fab Fragment (VR0260) is specific for MERS-COV S recognition. The antibody can be used in applications: ELISA. The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. For any requirments, please specify and submit a inquiry for assistance.

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Product Summary
Product CategoryPrimary Antibody
TargetS
HostHuman
Species ReactivityMERS-COV
ClonalityMonoclonal
ApplicationELISA
Product Details
SpecificityThis antibody recognizes spike protein of MERS-COV.
CloneVR0260
IsotypeIgG
SubtypeFab
Antigen Details
Full NameSpike glycoprotein
IntroductionS glycoprotein, also konwn as spike protein, densely decorates the viral surface and is a key player in pathogenesis, it is the main target of neutralizing antibodies and the focus of intense interest for vaccine design.
UniProt IDA0A140AYZ5
Alternative NamesS, S glycoprotein, Spike, Spike glycoprotein, E2 glycoprotein precursor, MERS-COV, MERS-COV S, MERS-COV Spike,Middle east respiratory syndrome, Coronavirus S, Coronavirus Spike
Related DiseaseMiddle east respiratory syndrome
Virus Details
Virus FamilyCoronaviridae
Virus SpeciesMERS-COV
Virus ClassificationPositive-sense RNA Virus
Packaging, Storage & Formulations
PurificationPurified with Protein A or G affinity chromatography
StorageStore at 4°C for short term. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.
BufferPBS (pH 7.4), please specify if any other requirements.
FormLiquid and Lyophilized powder are both available.
PreservativePreservative-free, please specify if any other requirements.
FormulationPBS (pH 7.4) without any preservative, please specify if any other requirements.
Concentration1mg/mL, please specify if any other requirements.
Application Notes
ApplicationELISA
Application NotesThe optimal working dilutions should be determined by the end user.
All products and services are intended for Research Use Only, and NOT to be used in diagnostic or therapeutic procedures.
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ADCCAntibody-dependent Cellular Cytotoxicity Assay
ApopApoptosis Assay
BABioAssay
BIBioimaging
BlockBlocking
CACell-based Assay/Cellular Assay
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CDCComplement-dependent Cytotoxicity Assay
CostimCostimulation Assay
CytCytotoxicity Assay
DepDepletion Assay
DBDot Blot/Slot blot
ECLElectrochemiluminescence
EMElectron Microscopy
EMSAElectrophoretic Mobility Shift Assay
ELISAEnzyme-Linked Immunosorbent Assay
ELISpotEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FIAFluorescence Immunoassay
FRETFluorescence Resonance Energy Transfer Microscopy
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination Assay
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IEPImmunoelectrophoresis
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology-Resin Sections
IMImmunomicroscopy
IPImmunoprecipitation
IRMAImmunoradiometric Assay
ISHIn Situ Hybridization
InhibInhibition Assay
ICFCIntracellular Staining for Flow Cytometry
Live-cell imagingLive-cell Imaging Assay
CyTOFMass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MAMicroagglutination Assay
NeutNeutralization Assay
PAPeptide Array
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation Assay
SPRSurface Plasmon Resonance
TCAT-Cell Activation Assay
TRFTime-Resolved Fluorescence
TCTissue Culture
TBTurbidimetry
WBWestern Blot
HIHemagglutinin Inhibition
FLISAFluorescence-linked immunosorbent assay
CtrlControl
Dual ISH-IHCDual In Situ Hybridization-Immunohistochemistry
ICFCIntracellular Staining for Flow Cytometry
KDKnockdown Validated
BLIBio-Layer Interferometry
LFIALateral-Flow Immunochromatographic Assay
CryCrystallization
LFALateral flow assay

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